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Assay Detail

CHEMBL648845

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
In vitro antagonistic activity for AT1 receptor by displacing 125I[Sar, ILe8]AII radioligand in rabbit aorta membrane using 0.2% bovine serum albumin (BSA)
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Oryctolagus cuniculus
Tissue Aorta
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Type-1 angiotensin II receptor (CHEMBL3948)
Type SINGLE PROTEIN
Organism Oryctolagus cuniculus

Publication

Triazolinones as nonpeptide angiotensin II antagonists. 1. Synthesis and evaluation of potent 2,4,5-trisubstituted triazolinones.
Chang LL, Ashton WT, Flanagan KL, Strelitz RA, MacCoss M, Greenlee WJ, Chang RS, Lotti VJ, Faust KA, Chen TB.
J Med Chem (1993) 36 : 2558 -2568
PubMed 8355255 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 7.96 8.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL87778 1 8.74
CHEMBL191 LOSARTAN 4.0 1 7.72
CHEMBL907 CARBOXYLIC ACID METABOLITE 1 7.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL87778 IC50 = 1.8 nM 8.74
CHEMBL191 LOSARTAN IC50 = 19.0 nM 7.72
CHEMBL907 CARBOXYLIC ACID METABOLITE IC50 = 37.0 nM 7.43