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Assay Detail

CHEMBL648846

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
In vitro antagonistic activity for AT1 receptor by displacing 125I[Sar, ILe8]AII radioligand in rabbit aorta membrane without using bovine serum albumin (BSA)
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Oryctolagus cuniculus
Tissue Aorta
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Type-1 angiotensin II receptor (CHEMBL3948)
Type SINGLE PROTEIN
Organism Oryctolagus cuniculus

Publication

Triazolinones as nonpeptide angiotensin II antagonists. 1. Synthesis and evaluation of potent 2,4,5-trisubstituted triazolinones.
Chang LL, Ashton WT, Flanagan KL, Strelitz RA, MacCoss M, Greenlee WJ, Chang RS, Lotti VJ, Faust KA, Chen TB.
J Med Chem (1993) 36 : 2558 -2568
PubMed 8355255 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.82 8.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL87964 1 8.68
CHEMBL422593 1 8.34
CHEMBL87778 1 8.30
CHEMBL314590 1 7.85
CHEMBL315128 1 7.85
CHEMBL87392 1 7.68
CHEMBL85704 1 7.30
CHEMBL86143 1 7.30
CHEMBL313017 1 7.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL87964 IC50 = 2.1 nM 8.68
CHEMBL422593 IC50 = 4.6 nM 8.34
CHEMBL87778 IC50 = 5.0 nM 8.30
CHEMBL314590 IC50 = 14.0 nM 7.85
CHEMBL315128 IC50 = 14.0 nM 7.85
CHEMBL87392 IC50 = 21.0 nM 7.68
CHEMBL85704 IC50 = 50.0 nM 7.30
CHEMBL86143 IC50 = 50.0 nM 7.30
CHEMBL313017 IC50 = 90.0 nM 7.05