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Assay Detail

CHEMBL650213

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Stimulation of cyclic AMP accumulation in C6 glioma cells expressing adrenergic beta receptor
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type C6
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Adrenergic receptor beta (CHEMBL2095166)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

Syntheses of 2,5- and 2,6-difluoronorepinephrine, 2,5-difluoroepinephrine, and 2,6-difluorophenylephrine: effect of disubstitution with fluorine on adrenergic activity.
Chen GT, King M, Gusovsky F, Creveling CR, Daly JW, Chen BH, Nie JY, Kirk KL.
J Med Chem (1993) 36 : 3947 -3955
PubMed 8254623 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 7 7.13 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL43711 1 7.40
CHEMBL1437 NOREPINEPHRINE 4.0 1 7.28
CHEMBL287587 1 7.20
CHEMBL679 EPINEPHRINE 4.0 1 6.64
CHEMBL281232 1 -
CHEMBL42359 1 -
CHEMBL435802 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL43711 EC50 = 40.0 nM 7.40
CHEMBL1437 NOREPINEPHRINE EC50 = 52.0 nM 7.28
CHEMBL287587 EC50 = 63.0 nM 7.20
CHEMBL679 EPINEPHRINE EC50 = 230.0 nM 6.64
CHEMBL281232 EC50 > 10000.0 nM -
CHEMBL42359 EC50 > 1500.0 nM -
CHEMBL435802 EC50 > 100000.0 nM -