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Assay Detail

CHEMBL661144

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Binding
In vitro cytochrome P450 17A1 inhibition was assayed using the rapid acetic acid releasing assay (AARA), utilizing intact P450c17-expressing Escherichia coli or P450c17-LNCaP cells as the enzyme source.
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type LNCaP
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Steroid 17-alpha-hydroxylase/17,20 lyase (CHEMBL3522)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Three dimensional pharmacophore modeling of human CYP17 inhibitors. Potential agents for prostate cancer therapy.
Clement OO, Freeman CM, Hartmann RW, Handratta VD, Vasaitis TS, Brodie AM, Njar VC.
J Med Chem (2003) 46 : 2345 -2351
PubMed 12773039 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 6.16 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL74661 1 7.30
CHEMBL1170 TESTOSTERONE PROPIONATE 4.0 1 7.25
CHEMBL310336 1 6.82
CHEMBL77751 NRB 03731 1 6.75
CHEMBL305896 1 6.65
CHEMBL75474 1 6.52
CHEMBL2112282 1 6.50
CHEMBL78108 1 6.42
CHEMBL78003 1 6.41
CHEMBL77288 1 6.35
CHEMBL77062 1 6.30
CHEMBL76877 1 6.25
CHEMBL80216 1 6.21
CHEMBL305941 1 6.20
CHEMBL419831 1 6.18
CHEMBL310822 1 6.10
CHEMBL254328 ABIRATERONE 3.0 1 6.10
CHEMBL77789 1 6.04
CHEMBL78215 1 6.00
CHEMBL307729 1 5.92
CHEMBL78222 1 5.90
CHEMBL80388 1 5.85
CHEMBL2112280 1 5.77
CHEMBL309691 1 5.58
CHEMBL307050 1 5.43
CHEMBL74606 1 5.42
CHEMBL78063 1 5.23
CHEMBL2112281 1 4.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL74661 IC50 = 50.0 nM 7.30
CHEMBL1170 TESTOSTERONE PROPIONATE IC50 = 56.0 nM 7.25
CHEMBL310336 IC50 = 150.0 nM 6.82
CHEMBL77751 NRB 03731 IC50 = 178.2 nM 6.75
CHEMBL305896 IC50 = 225.0 nM 6.65
CHEMBL75474 IC50 = 300.0 nM 6.52
CHEMBL2112282 IC50 = 320.0 nM 6.50
CHEMBL78108 IC50 = 377.3 nM 6.42
CHEMBL78003 IC50 = 386.9 nM 6.41
CHEMBL77288 IC50 = 450.0 nM 6.35
CHEMBL77062 IC50 = 500.0 nM 6.30
CHEMBL76877 IC50 = 562.0 nM 6.25
CHEMBL80216 IC50 = 616.5 nM 6.21
CHEMBL305941 IC50 = 628.0 nM 6.20
CHEMBL419831 IC50 = 660.25 nM 6.18
CHEMBL310822 IC50 = 800.0 nM 6.10
CHEMBL254328 ABIRATERONE IC50 = 800.0 nM 6.10
CHEMBL77789 IC50 = 915.0 nM 6.04
CHEMBL78215 IC50 = 1000.0 nM 6.00
CHEMBL307729 IC50 = 1200.0 nM 5.92
CHEMBL78222 IC50 = 1250.0 nM 5.90
CHEMBL80388 IC50 = 1400.0 nM 5.85
CHEMBL2112280 IC50 = 1700.0 nM 5.77
CHEMBL309691 IC50 = 2600.0 nM 5.58
CHEMBL307050 IC50 = 3700.0 nM 5.43
CHEMBL74606 IC50 = 3810.0 nM 5.42
CHEMBL78063 IC50 = 5900.0 nM 5.23
CHEMBL2112281 IC50 = 12050.0 nM 4.92