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Assay Detail

CHEMBL664922

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity against dihydrofolate reductase DHFR in Toxoplasma gondii.
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Toxoplasma gondii
Confidence 9 — Direct single protein target
Curated By Expert

Target

Bifunctional dihydrofolate reductase-thymidylate synthase (CHEMBL2425)
Type SINGLE PROTEIN
Organism Toxoplasma gondii

Publication

Structural studies on bioactive compounds. 34. Design, synthesis, and biological evaluation of triazenyl-substituted pyrimethamine inhibitors of Pneumocystis carinii dihydrofolate reductase.
Chan DC, Laughton CA, Queener SF, Stevens MF.
J Med Chem (2001) 44 : 2555 -2564
PubMed 11472209 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.13 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL119 TRIMETREXATE 4.0 1 8.00
CHEMBL87383 1 7.16
CHEMBL313034 1 7.10
CHEMBL313553 1 6.70
CHEMBL87013 1 6.66
CHEMBL49246 1 6.51
CHEMBL314001 1 6.42
CHEMBL36 PYRIMETHAMINE 4.0 1 6.41
CHEMBL313440 1 6.36
CHEMBL85188 1 6.20
CHEMBL87730 1 6.19
CHEMBL85576 1 6.18
CHEMBL55764 1 6.16
CHEMBL314387 1 6.16
CHEMBL444587 1 6.10
CHEMBL88004 1 6.08
CHEMBL313131 1 6.08
CHEMBL85958 1 6.07
CHEMBL313680 1 5.98
CHEMBL85573 1 5.94
CHEMBL85875 1 5.90
CHEMBL2115210 1 5.83
CHEMBL82853 1 5.75
CHEMBL86085 1 5.70
CHEMBL87423 1 5.59
CHEMBL2114205 1 5.57
CHEMBL87549 1 5.36
CHEMBL315297 1 5.27
CHEMBL85919 1 5.21
CHEMBL441062 1 5.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL119 TRIMETREXATE IC50 = 10.0 nM 8.00
CHEMBL87383 IC50 = 70.0 nM 7.16
CHEMBL313034 IC50 = 80.0 nM 7.10
CHEMBL313553 IC50 = 200.0 nM 6.70
CHEMBL87013 IC50 = 220.0 nM 6.66
CHEMBL49246 IC50 = 310.0 nM 6.51
CHEMBL314001 IC50 = 378.0 nM 6.42
CHEMBL36 PYRIMETHAMINE IC50 = 390.0 nM 6.41
CHEMBL313440 IC50 = 440.0 nM 6.36
CHEMBL85188 IC50 = 630.0 nM 6.20
CHEMBL87730 IC50 = 640.0 nM 6.19
CHEMBL85576 IC50 = 660.0 nM 6.18
CHEMBL55764 IC50 = 690.0 nM 6.16
CHEMBL314387 IC50 = 700.0 nM 6.16
CHEMBL444587 IC50 = 800.0 nM 6.10
CHEMBL88004 IC50 = 830.0 nM 6.08
CHEMBL313131 IC50 = 830.0 nM 6.08
CHEMBL85958 IC50 = 850.0 nM 6.07
CHEMBL313680 IC50 = 1050.0 nM 5.98
CHEMBL85573 IC50 = 1160.0 nM 5.94
CHEMBL85875 IC50 = 1250.0 nM 5.90
CHEMBL2115210 IC50 = 1470.0 nM 5.83
CHEMBL82853 IC50 = 1790.0 nM 5.75
CHEMBL86085 IC50 = 2010.0 nM 5.70
CHEMBL87423 IC50 = 2540.0 nM 5.59
CHEMBL2114205 IC50 = 2700.0 nM 5.57
CHEMBL87549 IC50 = 4400.0 nM 5.36
CHEMBL315297 IC50 = 5390.0 nM 5.27
CHEMBL85919 IC50 = 6100.0 nM 5.21
CHEMBL441062 IC50 = 7800.0 nM 5.11