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Assay Detail

CHEMBL672256

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Receptor binding affinity was determined against [125I]ET1 with membranes prepared from human placenta for ETB receptor
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Endothelin receptor type B (CHEMBL1785)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Ro 48-5695: A potent mixed endothelin receptor antagonist optimized from bosentan
Neidhart W, Breu V, Burri K, Clozel M, Hirth G, Klinkhammer U, Giller T, Ramuz H
Bioorg Med Chem Lett (1997) 7 : 2223 -2228
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.05 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL437472 ENDOTHELIN 2.0 1 9.70
CHEMBL72037 1 8.30
CHEMBL308088 1 7.85
CHEMBL70339 1 7.72
CHEMBL70385 1 7.60
CHEMBL70895 1 7.60
CHEMBL70203 1 7.37
CHEMBL70293 1 7.31
CHEMBL70487 1 7.22
CHEMBL303428 1 7.20
CHEMBL72473 1 7.16
CHEMBL70800 1 6.92
CHEMBL303649 1 6.84
CHEMBL957 BOSENTAN 4.0 1 6.82
CHEMBL71070 1 6.80
CHEMBL70303 1 6.77
CHEMBL70365 1 6.70
CHEMBL435895 1 6.52
CHEMBL70788 1 6.16
CHEMBL70781 1 6.11
CHEMBL74118 1 6.00
CHEMBL70924 1 5.91
CHEMBL307803 1 5.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL437472 ENDOTHELIN IC50 = 0.2 nM 9.70
CHEMBL72037 IC50 = 5.0 nM 8.30
CHEMBL308088 IC50 = 14.0 nM 7.85
CHEMBL70339 IC50 = 19.0 nM 7.72
CHEMBL70385 IC50 = 25.0 nM 7.60
CHEMBL70895 IC50 = 25.0 nM 7.60
CHEMBL70203 IC50 = 43.0 nM 7.37
CHEMBL70293 IC50 = 49.0 nM 7.31
CHEMBL70487 IC50 = 60.0 nM 7.22
CHEMBL303428 IC50 = 63.0 nM 7.20
CHEMBL72473 IC50 = 70.0 nM 7.16
CHEMBL70800 IC50 = 120.0 nM 6.92
CHEMBL303649 IC50 = 144.0 nM 6.84
CHEMBL957 BOSENTAN IC50 = 150.0 nM 6.82
CHEMBL71070 IC50 = 160.0 nM 6.80
CHEMBL70303 IC50 = 170.0 nM 6.77
CHEMBL70365 IC50 = 200.0 nM 6.70
CHEMBL435895 IC50 = 300.0 nM 6.52
CHEMBL70788 IC50 = 700.0 nM 6.16
CHEMBL70781 IC50 = 770.0 nM 6.11
CHEMBL74118 IC50 = 1000.0 nM 6.00
CHEMBL70924 IC50 = 1240.0 nM 5.91
CHEMBL307803 IC50 = 2800.0 nM 5.55