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Assay Detail

CHEMBL672588

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Ability to inhibit binding of [3H]WIN-35428 to dopamine transporter in rat caudate putamen
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Caudate-putamen
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Sodium-dependent dopamine transporter (CHEMBL338)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Effects of two-carbon bridge region methoxylation of benztropine: discovery of novel chiral ligands for the dopamine transporter.
Simoni D, Roberti M, Rondanin R, Baruchello R, Rossi M, Invidiata FP, Merighi S, Varani K, Gessi S, Borea PA, Marino S, Cavallini S, Bianchi C, Siniscalchi A.
Bioorg Med Chem Lett (2001) 11 : 823 -827
PubMed 11277529 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.76 7.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL424565 1 7.60
CHEMBL168814 1 7.50
CHEMBL370805 COCAINE 4.0 1 7.05
CHEMBL355286 1 7.02
CHEMBL1201203 BENZTROPINE 4.0 1 6.93
CHEMBL355258 1 6.19
CHEMBL168371 1 6.12
CHEMBL3084973 1 5.70
CHEMBL3084972 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL424565 IC50 = 25.0 nM 7.60
CHEMBL168814 IC50 = 32.0 nM 7.50
CHEMBL370805 COCAINE IC50 = 89.0 nM 7.05
CHEMBL355286 IC50 = 95.0 nM 7.02
CHEMBL1201203 BENZTROPINE IC50 = 118.0 nM 6.93
CHEMBL355258 IC50 = 650.0 nM 6.19
CHEMBL168371 IC50 = 750.0 nM 6.12
CHEMBL3084973 IC50 = 2000.0 nM 5.70
CHEMBL3084972 IC50 > 10000.0 nM -