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Assay Detail

CHEMBL672809

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro effective concentration tested on HEK293 cells co-transfected with rat Dopamine receptor D2 using FLIPR assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HEK293
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

D(2) dopamine receptor (CHEMBL339)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Discovery of 2-(4-pyridin-2-ylpiperazin-1-ylmethyl)-1H-benzimidazole (ABT-724), a dopaminergic agent with a novel mode of action for the potential treatment of erectile dysfunction.
Cowart M, Latshaw SP, Bhatia P, Daanen JF, Rohde J, Nelson SL, Patel M, Kolasa T, Nakane M, Uchic ME, Miller LN, Terranova MA, Chang R, Donnelly-Roberts DL, Namovic MT, Hollingsworth PR, Martino BR, Lynch JJ, Sullivan JP, Hsieh GC, Moreland RB, Brioni JD, Stewart AO.
J Med Chem (2004) 47 : 3853 -3864
PubMed 15239663 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 2 8.11 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL59 DOPAMINE 4.0 1 8.96
CHEMBL77395 1 7.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL59 DOPAMINE EC50 = 1.1 nM 8.96
CHEMBL77395 EC50 = 55.0 nM 7.26