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Assay Detail

CHEMBL674648

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Binding
Inhibit of phosphorylation of a tyrosine/glutamic acid copolymer by epidermal growth factor receptor prepared from human A431 carcinoma cell vesicles by immunoaffinity chromatography.
32
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tyrosine kinase inhibitors. 14. Structure-activity relationships for methylamino-substituted derivatives of 4-[(3-bromophenyl)amino]-6-(methylamino)-pyrido[3,4-d]pyrimidine (PD 158780), a potent and specific inhibitor of the tyrosine kinase activity of receptors for the EGF family of growth factors.
Rewcastle GW, Murray DK, Elliott WL, Fry DW, Howard CT, Nelson JM, Roberts BJ, Vincent PW, Showalter HD, Winters RT, Denny WA.
J Med Chem (1998) 41 : 742 -751
PubMed 9513602 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 32 8.88 9.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL51853 1 9.89
CHEMBL166093 1 9.74
CHEMBL166154 1 9.72
CHEMBL165864 1 9.72
CHEMBL161956 1 9.66
CHEMBL355816 1 9.55
CHEMBL163369 1 9.46
CHEMBL351629 1 9.36
CHEMBL165547 1 9.25
CHEMBL162622 1 9.19
CHEMBL162142 1 9.11
CHEMBL165630 1 9.03
CHEMBL165574 1 9.00
CHEMBL165788 1 8.96
CHEMBL166358 1 8.92
CHEMBL162223 1 8.92
CHEMBL161895 1 8.89
CHEMBL165731 1 8.82
CHEMBL165508 1 8.82
CHEMBL162981 1 8.77
CHEMBL166255 1 8.77
CHEMBL349070 1 8.74
CHEMBL162508 1 8.74
CHEMBL163188 1 8.55
CHEMBL165495 1 8.51
CHEMBL166307 1 8.41
CHEMBL162719 1 8.19
CHEMBL162053 1 8.09
CHEMBL53940 1 8.05
CHEMBL163168 1 7.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL51853 IC50 = 0.13 nM 9.89
CHEMBL166093 IC50 = 0.18 nM 9.74
CHEMBL166154 IC50 = 0.19 nM 9.72
CHEMBL165864 IC50 = 0.19 nM 9.72
CHEMBL161956 IC50 = 0.22 nM 9.66
CHEMBL355816 IC50 = 0.28 nM 9.55
CHEMBL163369 IC50 = 0.35 nM 9.46
CHEMBL351629 IC50 = 0.44 nM 9.36
CHEMBL165547 IC50 = 0.56 nM 9.25
CHEMBL162622 IC50 = 0.65 nM 9.19
CHEMBL162142 IC50 = 0.78 nM 9.11
CHEMBL165630 IC50 = 0.93 nM 9.03
CHEMBL165574 IC50 = 1.0 nM 9.00
CHEMBL165788 IC50 = 1.1 nM 8.96
CHEMBL162223 IC50 = 1.2 nM 8.92
CHEMBL166358 IC50 = 1.2 nM 8.92
CHEMBL161895 IC50 = 1.3 nM 8.89
CHEMBL165731 IC50 = 1.5 nM 8.82
CHEMBL165508 IC50 = 1.5 nM 8.82
CHEMBL162981 IC50 = 1.7 nM 8.77
CHEMBL166255 IC50 = 1.7 nM 8.77
CHEMBL349070 IC50 = 1.8 nM 8.74
CHEMBL162508 IC50 = 1.8 nM 8.74
CHEMBL163188 IC50 = 2.8 nM 8.55
CHEMBL165495 IC50 = 3.1 nM 8.51
CHEMBL166307 IC50 = 3.9 nM 8.41
CHEMBL162719 IC50 = 6.4 nM 8.19
CHEMBL162053 IC50 = 8.1 nM 8.09
CHEMBL53940 IC50 = 9.0 nM 8.05
CHEMBL163168 IC50 = 27.0 nM 7.57
CHEMBL165228 IC50 = 117.0 nM 6.93
CHEMBL53711 IC50 = 0.006 nM -