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Assay Detail

CHEMBL677211

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [gamma-32P]-ATP from Epidermal growth factor receptor in A431 cell-free extracts
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A-431
Confidence 9 — Direct single protein target
Curated By Expert

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological activity of 5-[(2,5-dihydroxybenzyl)amino]salicylic acid analogs as inhibitors of EGF receptor-associated protein tyrosine kinase
Liu T, Shirai R, Matsui T, Umezawa K, Iwasaki S
Bioorg Med Chem Lett (1997) 7 : 365 -368
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.10 6.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL122397 1 6.05
CHEMBL319620 LAVENDUSTIN C 1 5.40
CHEMBL123045 1 5.27
CHEMBL122260 1 5.01
CHEMBL122785 1 4.99
CHEMBL122721 1 4.57
CHEMBL123046 1 4.38

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL122397 IC50 = 900.0 nM 6.05
CHEMBL319620 LAVENDUSTIN C IC50 = 4000.0 nM 5.40
CHEMBL123045 IC50 = 5400.0 nM 5.27
CHEMBL122260 IC50 = 9700.0 nM 5.01
CHEMBL122785 IC50 = 10300.0 nM 4.99
CHEMBL122721 IC50 = 27200.0 nM 4.57
CHEMBL123046 IC50 = 41900.0 nM 4.38