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Assay Detail

CHEMBL679119

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibition of endothelin binding to endothelin A receptor in rat heart ventricles was measured using a 125-I labelled ET-1 competition assay
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Endothelin-1 receptor (CHEMBL4566)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Nonpeptide endothelin antagonists: from lower affinity pyrazol-5-ols to higher affinity pyrazole-5-carboxylic acids.
Zhang J, Didierlaurent S, Fortin M, Lefrançois D, Uridat E, Vevert JP.
Bioorg Med Chem Lett (2000) 10 : 1351 -1355
PubMed 10890162 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 5.58 7.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL26474 1 7.75
CHEMBL281865 1 7.55
CHEMBL3144737 1 6.96
CHEMBL3144607 1 6.85
CHEMBL3144732 1 6.38
CHEMBL281677 1 6.35
CHEMBL3144605 1 6.22
CHEMBL3144735 1 5.89
CHEMBL3144599 1 5.82
CHEMBL3144606 1 5.77
CHEMBL3144729 1 5.75
CHEMBL3144806 1 5.72
CHEMBL3144799 1 5.68
CHEMBL3144850 1 5.51
CHEMBL3144800 1 5.46
CHEMBL3144733 1 5.42
CHEMBL3144600 1 5.42
CHEMBL3144604 1 5.37
CHEMBL3144602 1 5.25
CHEMBL3144728 1 5.12
CHEMBL3144803 1 5.00
CHEMBL3144845 1 5.00
CHEMBL3144846 1 4.70
CHEMBL283037 1 4.40
CHEMBL3144603 1 4.40
CHEMBL3144736 1 4.32
CHEMBL444545 1 4.19
CHEMBL3144831 1 4.01

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL26474 IC50 = 18.0 nM 7.75
CHEMBL281865 IC50 = 28.0 nM 7.55
CHEMBL3144737 IC50 = 110.0 nM 6.96
CHEMBL3144607 IC50 = 140.0 nM 6.85
CHEMBL3144732 IC50 = 420.0 nM 6.38
CHEMBL281677 IC50 = 450.0 nM 6.35
CHEMBL3144605 IC50 = 600.0 nM 6.22
CHEMBL3144735 IC50 = 1300.0 nM 5.89
CHEMBL3144599 IC50 = 1500.0 nM 5.82
CHEMBL3144606 IC50 = 1700.0 nM 5.77
CHEMBL3144729 IC50 = 1800.0 nM 5.75
CHEMBL3144806 IC50 = 1900.0 nM 5.72
CHEMBL3144799 IC50 = 2100.0 nM 5.68
CHEMBL3144850 IC50 = 3100.0 nM 5.51
CHEMBL3144800 IC50 = 3500.0 nM 5.46
CHEMBL3144733 IC50 = 3800.0 nM 5.42
CHEMBL3144600 IC50 = 3800.0 nM 5.42
CHEMBL3144604 IC50 = 4300.0 nM 5.37
CHEMBL3144602 IC50 = 5600.0 nM 5.25
CHEMBL3144728 IC50 = 7500.0 nM 5.12
CHEMBL3144803 IC50 = 10000.0 nM 5.00
CHEMBL3144845 IC50 = 10000.0 nM 5.00
CHEMBL3144846 IC50 = 20000.0 nM 4.70
CHEMBL283037 IC50 = 40000.0 nM 4.40
CHEMBL3144603 IC50 = 40000.0 nM 4.40
CHEMBL3144736 IC50 = 48000.0 nM 4.32
CHEMBL444545 IC50 = 64000.0 nM 4.19
CHEMBL3144831 IC50 = 98000.0 nM 4.01