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Assay Detail

CHEMBL686439

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Binding
In vitro binding assay of Shc-derived phosphopeptide to Growth factor receptor bound protein 2 SH2 in B104-1-1 cells was determined
16
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type B104-1-1
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Growth factor receptor-bound protein 2 (CHEMBL4830)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Natural and synthetic analogues of actinomycin D as Grb2-SH2 domain blockers.
Kim HK, Nam JY, Han MY, Son KH, Choi JD, Kwon BM, Takusagawa HL, Huang Y, Takusagawa F.
Bioorg Med Chem Lett (2000) 10 : 1455 -1457
PubMed 10888331 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.75 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2369548 2 6.30
CHEMBL2369547 2 6.10
CHEMBL2369549 2 6.10
CHEMBL2372234 1 6.05
CHEMBL2372235 1 6.00
CHEMBL2372236 1 5.92
CHEMBL2372231 1 5.82
CHEMBL2372240 1 5.75
CHEMBL2372239 1 5.70
CHEMBL2372241 1 5.70
CHEMBL1554 DACTINOMYCIN 4.0 1 5.30
CHEMBL2369551 1 5.23
CHEMBL2369550 1 5.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2369548 IC50 = 500.0 nM 6.30
CHEMBL2369547 IC50 = 800.0 nM 6.10
CHEMBL2369549 IC50 = 800.0 nM 6.10
CHEMBL2372234 IC50 = 900.0 nM 6.05
CHEMBL2372235 IC50 = 1000.0 nM 6.00
CHEMBL2372236 IC50 = 1200.0 nM 5.92
CHEMBL2372231 IC50 = 1500.0 nM 5.82
CHEMBL2372240 IC50 = 1800.0 nM 5.75
CHEMBL2372239 IC50 = 2000.0 nM 5.70
CHEMBL2372241 IC50 = 2000.0 nM 5.70
CHEMBL2369548 IC50 = 4500.0 nM 5.35
CHEMBL1554 DACTINOMYCIN IC50 = 5000.0 nM 5.30
CHEMBL2369551 IC50 = 5900.0 nM 5.23
CHEMBL2369550 IC50 = 7600.0 nM 5.12
CHEMBL2369549 IC50 > 10000.0 nM -
CHEMBL2369547 IC50 > 10000.0 nM -