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Assay Detail

CHEMBL687578

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Concentration producing 50% of the maximal increase in the force of contraction in isolated guinea pig left atrium
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Cavia porcellus
Tissue Cardiac atrium
Confidence 1 — Organism
Curated By Expert

Target

Cavia porcellus (CHEMBL369)
Type ORGANISM
Organism Cavia porcellus

Publication

17beta-O-Aminoalkyloximes of 5beta-androstane-3beta,14beta-diol with digitalis-like activity: synthesis, cardiotonic activity, structure-activity relationships, and molecular modeling of the Na(+),K(+)-ATPase receptor.
Cerri A, Almirante N, Barassi P, Benicchio A, Fedrizzi G, Ferrari P, Micheletti R, Quadri L, Ragg E, Rossi R, Santagostino M, Schiavone A, Serra F, Zappavigna MP, Melloni P.
J Med Chem (2000) 43 : 2332 -2349
PubMed 10882359 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 28 5.91 7.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2068972 1 7.46
CHEMBL2068887 1 7.30
CHEMBL2068896 1 7.24
CHEMBL2069110 1 7.16
CHEMBL2068909 1 7.16
CHEMBL2068904 1 6.77
CHEMBL2068894 1 6.75
CHEMBL1751 DIGOXIN 4.0 1 6.42
CHEMBL2069040 1 6.42
CHEMBL1453 DIGITOXIGENIN 1 6.24
CHEMBL2068901 1 6.21
CHEMBL2069059 1 6.19
CHEMBL2068983 1 5.96
CHEMBL2068979 1 5.89
CHEMBL2068897 1 5.75
CHEMBL2068895 1 5.54
CHEMBL2069060 1 5.41
CHEMBL2068890 1 5.40
CHEMBL2069048 1 5.40
CHEMBL2068984 1 5.33
CHEMBL2069061 1 5.10
CHEMBL2068981 1 4.96
CHEMBL2068978 1 4.92
CHEMBL2068910 1 4.75
CHEMBL2069058 1 4.70
CHEMBL2068893 1 4.64
CHEMBL2069044 1 4.41
CHEMBL2068902 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2068972 EC50 = 35.0 nM 7.46
CHEMBL2068887 EC50 = 50.0 nM 7.30
CHEMBL2068896 EC50 = 57.0 nM 7.24
CHEMBL2069110 EC50 = 70.0 nM 7.16
CHEMBL2068909 EC50 = 70.0 nM 7.16
CHEMBL2068904 EC50 = 170.0 nM 6.77
CHEMBL2068894 EC50 = 180.0 nM 6.75
CHEMBL1751 DIGOXIN EC50 = 380.0 nM 6.42
CHEMBL2069040 EC50 = 380.0 nM 6.42
CHEMBL1453 DIGITOXIGENIN EC50 = 570.0 nM 6.24
CHEMBL2068901 EC50 = 620.0 nM 6.21
CHEMBL2069059 EC50 = 650.0 nM 6.19
CHEMBL2068983 EC50 = 1100.0 nM 5.96
CHEMBL2068979 EC50 = 1300.0 nM 5.89
CHEMBL2068897 EC50 = 1800.0 nM 5.75
CHEMBL2068895 EC50 = 2900.0 nM 5.54
CHEMBL2069060 EC50 = 3900.0 nM 5.41
CHEMBL2068890 EC50 = 4000.0 nM 5.40
CHEMBL2069048 EC50 = 4000.0 nM 5.40
CHEMBL2068984 EC50 = 4700.0 nM 5.33
CHEMBL2069061 EC50 = 8000.0 nM 5.10
CHEMBL2068981 EC50 = 11000.0 nM 4.96
CHEMBL2068978 EC50 = 12000.0 nM 4.92
CHEMBL2068910 EC50 = 18000.0 nM 4.75
CHEMBL2069058 EC50 = 20000.0 nM 4.70
CHEMBL2068893 EC50 = 23000.0 nM 4.64
CHEMBL2069044 EC50 = 39000.0 nM 4.41
CHEMBL2068902 EC50 = - nM -