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Assay Detail

CHEMBL688360

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Tested for the cytotoxicity to inhibit replication assay against the human colon tumor cell line HCT116
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HCT-116
Confidence 1 — Organism
Curated By Intermediate

Target

HCT-116 (CHEMBL394)
Type CELL-LINE
Organism Homo sapiens

Publication

Inhibition of topoisomerase II catalytic activity by pyridoacridine alkaloids from a Cystodytes sp. ascidian: a mechanism for the apparent intercalator-induced inhibition of topoisomerase II.
McDonald LA, Eldredge GS, Barrows LR, Ireland CM.
J Med Chem (1994) 37 : 3819 -3827
PubMed 7525959 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.23 5.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL339820 1 5.80
CHEMBL44657 ETOPOSIDE 4.0 1 5.60
CHEMBL127811 EILATIN 1 5.28
CHEMBL43 AMSACRINE 4.0 1 5.20
CHEMBL127872 KUANONIAMINE D 1 5.11
CHEMBL127873 SHERMILAMINE B 1 4.86
CHEMBL127939 1 4.79
CHEMBL124579 DIPLAMINE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL339820 IC50 = 1600.0 nM 5.80
CHEMBL44657 ETOPOSIDE IC50 = 2500.0 nM 5.60
CHEMBL127811 EILATIN IC50 = 5300.0 nM 5.28
CHEMBL43 AMSACRINE IC50 = 6300.0 nM 5.20
CHEMBL127872 KUANONIAMINE D IC50 = 7800.0 nM 5.11
CHEMBL127873 SHERMILAMINE B IC50 = 13800.0 nM 4.86
CHEMBL127939 IC50 = 16300.0 nM 4.79
CHEMBL124579 DIPLAMINE IC50 < 1400.0 nM -