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Assay Detail

CHEMBL696376

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of specific binding of [3H]kainate to Ionotropic glutamate receptor kainate in rat brain membranes
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Brain
Subcellular Brain membranes
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Glutamate receptor ionotropic, kainate (CHEMBL2094119)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Structure--activity studies for alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropanoic acid receptors: acidic hydroxyphenylalanines.
Hill RA, Wallace LJ, Miller DD, Weinstein DM, Shams G, Tai H, Layer RT, Willins D, Uretsky NJ, Danthi SN.
J Med Chem (1997) 40 : 3182 -3191
PubMed 9379437 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.46 8.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL275040 KAINIC ACID 2.0 1 8.27
CHEMBL575060 GLUTAMIC ACID 3.0 1 6.16
CHEMBL155265 DNQX 1 5.66
CHEMBL9790 1 5.62
CHEMBL366588 1 5.43
CHEMBL108147 1 4.47
CHEMBL276815 (S)-AMPA 1 4.04
CHEMBL366489 1 4.01
CHEMBL427991 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL275040 KAINIC ACID IC50 = 5.4 nM 8.27
CHEMBL575060 GLUTAMIC ACID IC50 = 700.0 nM 6.16
CHEMBL155265 DNQX IC50 = 2200.0 nM 5.66
CHEMBL9790 IC50 = 2400.0 nM 5.62
CHEMBL366588 IC50 = 3700.0 nM 5.43
CHEMBL108147 IC50 = 34000.0 nM 4.47
CHEMBL276815 (S)-AMPA IC50 = 92000.0 nM 4.04
CHEMBL366489 IC50 = 97000.0 nM 4.01
CHEMBL427991 IC50 > 100000.0 nM -