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Assay Detail

CHEMBL697515

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Effective concentration for MIPA (muscarinic-stimulated inositol phosphate accumulation) activity in SK-N-SH neuroblastoma cells expressing human muscarinic M3 receptor
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type SK-N-SH
Confidence 9 — Direct single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M3 (CHEMBL245)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A rationale for the design and synthesis of m1 selective muscarinic agonists.
Tecle H, Mirzadegan T, Moos W, Moreland D, Pavia M, Schwarz R, Davis R
Bioorg Med Chem Lett (1992) 2 : 821 -826
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 4.97 5.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL315676 1 5.08
CHEMBL354738 1 5.06
CHEMBL352908 1 5.05
CHEMBL168545 1 5.02
CHEMBL14 CARBACHOL 4.0 1 4.97
CHEMBL85678 1 4.84
CHEMBL313918 1 4.79
CHEMBL314737 OXADIAZOLE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL315676 EC50 = 8400.0 nM 5.08
CHEMBL354738 EC50 = 8800.0 nM 5.06
CHEMBL352908 EC50 = 8900.0 nM 5.05
CHEMBL168545 EC50 = 9600.0 nM 5.02
CHEMBL14 CARBACHOL EC50 = 10600.0 nM 4.97
CHEMBL85678 EC50 = 14600.0 nM 4.84
CHEMBL313918 EC50 = 16400.0 nM 4.79
CHEMBL314737 OXADIAZOLE EC50 = 104000.0 nM -