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Assay Detail

CHEMBL710823

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 2-[125I]iodomelatonin binding to human Melatonin receptor type 1B expressed in HEK293 cells
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Melatonin receptor type 1B (CHEMBL1946)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of nitroindole derivatives with high affinity and selectivity for melatoninergic binding sites MT(3).
Leclerc V, Yous S, Delagrange P, Boutin JA, Renard P, Lesieur D.
J Med Chem (2002) 45 : 1853 -1859
PubMed 11960497 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 8.39 10.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL300056 1 10.33
CHEMBL289233 IODOMELATONIN 1 9.80
CHEMBL52755 1 9.77
CHEMBL45 MELATONIN 4.0 1 9.51
CHEMBL55084 1 9.11
CHEMBL33700 1 8.51
CHEMBL298335 1 8.28
CHEMBL52559 1 7.62
CHEMBL300729 1 7.54
CHEMBL300728 1 7.41
CHEMBL53622 1 6.71
CHEMBL300195 1 6.09

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL300056 Ki = 0.047 nM 10.33
CHEMBL289233 IODOMELATONIN Ki = 0.16 nM 9.80
CHEMBL52755 Ki = 0.17 nM 9.77
CHEMBL45 MELATONIN Ki = 0.31 nM 9.51
CHEMBL55084 Ki = 0.77 nM 9.11
CHEMBL33700 Ki = 3.1 nM 8.51
CHEMBL298335 Ki = 5.3 nM 8.28
CHEMBL52559 Ki = 23.7 nM 7.62
CHEMBL300729 Ki = 29.0 nM 7.54
CHEMBL300728 Ki = 39.0 nM 7.41
CHEMBL53622 Ki = 194.0 nM 6.71
CHEMBL300195 Ki = 820.0 nM 6.09