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Assay Detail

CHEMBL745919

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity against Muscarinic receptor from rat brain membranes using [3H]pirenzepine
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M1 (CHEMBL276)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and biological evaluation of tropane-like 1-[2-[bis(4-fluorophenyl)methoxy]ethyl]-4-(3-phenylpropyl)piperazine (GBR 12909) analogues.
Zhang Y, Joseph DB, Bowen WD, Flippen-Anderson JL, Dersch CM, Rothman RB, Jacobson AE, Rice KC.
J Med Chem (2001) 44 : 3937 -3945
PubMed 11689080 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 6.68 9.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1201203 BENZTROPINE 4.0 1 9.23
CHEMBL3084922 1 7.25
CHEMBL3084920 1 7.11
CHEMBL130643 1 7.03
CHEMBL126235 1 6.89
CHEMBL3084919 1 6.77
CHEMBL129193 1 6.70
CHEMBL126735 1 6.47
CHEMBL3084921 1 6.20
CHEMBL3084918 1 5.92
CHEMBL127053 1 5.92
CHEMBL415721 1 5.77
CHEMBL339614 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1201203 BENZTROPINE Ki = 0.59 nM 9.23
CHEMBL3084922 Ki = 56.0 nM 7.25
CHEMBL3084920 Ki = 78.0 nM 7.11
CHEMBL130643 Ki = 94.0 nM 7.03
CHEMBL126235 Ki = 130.0 nM 6.89
CHEMBL3084919 Ki = 170.0 nM 6.77
CHEMBL129193 Ki = 200.0 nM 6.70
CHEMBL126735 Ki = 340.0 nM 6.47
CHEMBL3084921 Ki = 630.0 nM 6.20
CHEMBL3084918 Ki = 1200.0 nM 5.92
CHEMBL127053 Ki = 1200.0 nM 5.92
CHEMBL415721 Ki = 1700.0 nM 5.77
CHEMBL339614 Ki = 2500.0 nM 5.60