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Assay Detail

CHEMBL746419

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Compound was tested for its ability to stimulate phosphoinositol (PI) hydrolysis in the mouse fibroblast cell line A9L-M1 expressing Muscarinic acetylcholine receptor M1 (100 uM concentration)
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type Fibroblast
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M1 (CHEMBL216)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1-(1,2,5-Thiadiazol-4-yl)-4-azatricyclo[2.2.1.0(2,6)]heptanes as new potent muscarinic M1 agonists: structure-activity relationship for 3-aryl-2-propyn-1-yloxy and 3-aryl-2-propyn-1-ylthio derivatives.
Jeppesen L, Olesen PH, Hansen L, Sheardown MJ, Thomsen C, Rasmussen T, Jensen AF, Christensen MS, Rimvall K, Ward JS, Whitesitt C, Calligaro DO, Bymaster FP, Delapp NW, Felder CC, Shannon HE, Sauerberg P.
J Med Chem (1999) 42 : 1999 -2006
PubMed 10354408 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 1 8.31 8.31

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL304247 1 8.31

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL304247 EC50 = 4.9 nM 8.31