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Assay Detail

CHEMBL747450

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-OXO-M (oxotremorine-M) from the central muscarinic receptor sites of the rat brain membranes
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Subcellular Membrane
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Muscarinic acetylcholine receptor (CHEMBL1907609)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

Synthesis and structure-activity relationships of heterocyclic analogues of the functional M1 selective muscarinic agonist hexyloxy-TZTP
Sauerberg P, Olesen PH, Suzdak PD, Sheardown MJ, Mitch CH, Sawyer BD, Shannon HE
Bioorg Med Chem Lett (1992) 2 : 809 -814
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.10 8.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL101054 1 8.85
CHEMBL21536 XANOMELINE 4.0 1 8.01
CHEMBL75907 1 7.62
CHEMBL74786 1 7.16
CHEMBL7303 ARECOLINE -1.0 1 7.11
CHEMBL74300 1 6.45
CHEMBL355465 1 6.32
CHEMBL353779 1 6.26
CHEMBL167375 1 6.12
CHEMBL168581 1 -
CHEMBL166115 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL101054 IC50 = 1.4 nM 8.85
CHEMBL21536 XANOMELINE IC50 = 9.7 nM 8.01
CHEMBL75907 IC50 = 24.0 nM 7.62
CHEMBL74786 IC50 = 70.0 nM 7.16
CHEMBL7303 ARECOLINE IC50 = 77.0 nM 7.11
CHEMBL74300 IC50 = 355.0 nM 6.45
CHEMBL355465 IC50 = 477.0 nM 6.32
CHEMBL353779 IC50 = 544.0 nM 6.26
CHEMBL167375 IC50 = 759.0 nM 6.12
CHEMBL168581 IC50 > 900.0 nM -
CHEMBL166115 IC50 > 900.0 nM -