Assay Detail
Binding
CHEMBL747778
Review assay metadata, readout intent, target linkage, and publication context from the same page.
The compound was tested in vitro for binding activity against M2 muscarinic receptor in homogenates of the brainstem of rat using [3H]quinuclidinyl benzilate (QNB) as radioligand
5
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Tissue | Brain |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M2 (CHEMBL309) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Synthesis and muscarinic M(2) subtype antagonistic activity of enantiomeric pairs of 3-demethylhimbacine (3-norhimbacine) and its C(4)-epimer.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 5 | 6.98 | 8.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL111224 | — | — | 2 | 8.10 |
| CHEMBL277642 | HIMBACINE | — | 1 | 7.90 |
| CHEMBL324725 | — | — | 2 | 6.40 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL111224 | — | Ki | = | 7.943 | nM | 8.10 |
| CHEMBL277642 | HIMBACINE | Ki | = | 12.59 | nM | 7.90 |
| CHEMBL324725 | — | Ki | = | 398.11 | nM | 6.40 |
| CHEMBL324725 | — | Ki | = | 398.11 | nM | 6.40 |
| CHEMBL111224 | — | Ki | = | 794.33 | nM | 6.10 |