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Assay Detail

CHEMBL748174

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]N-methylscopolamine binding to muscarinic acetylcholine receptor of rat cerebral cortex.
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Cerebral cortex
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Muscarinic acetylcholine receptor (CHEMBL1907609)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

Phenyl-substituted analogues of oxotremorine as muscarinic antagonists.
Nilsson BM, Vargas HM, Ringdahl B, Hacksell U.
J Med Chem (1992) 35 : 285 -294
PubMed 1732545 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 6.24 7.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL51405 1 7.25
CHEMBL310852 1 7.19
CHEMBL101645 1 6.96
CHEMBL97915 1 6.92
CHEMBL99266 1 6.64
CHEMBL100406 1 6.51
CHEMBL318049 1 6.27
CHEMBL319665 1 5.89
CHEMBL97890 1 5.62
CHEMBL99265 1 5.28
CHEMBL318528 1 5.24
CHEMBL101646 1 5.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL51405 Ki = 56.0 nM 7.25
CHEMBL310852 Ki = 64.0 nM 7.19
CHEMBL101645 Ki = 110.0 nM 6.96
CHEMBL97915 Ki = 120.0 nM 6.92
CHEMBL99266 Ki = 230.0 nM 6.64
CHEMBL100406 Ki = 310.0 nM 6.51
CHEMBL318049 Ki = 540.0 nM 6.27
CHEMBL319665 Ki = 1290.0 nM 5.89
CHEMBL97890 Ki = 2400.0 nM 5.62
CHEMBL99265 Ki = 5200.0 nM 5.28
CHEMBL318528 Ki = 5770.0 nM 5.24
CHEMBL101646 Ki = 6910.0 nM 5.16