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Assay Detail

CHEMBL748854

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]pirenzepine binding to muscarinic M1 receptor in brain cortex of rat.
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Brain
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M1 (CHEMBL276)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Quinolizidinyl derivatives of 5,11-dihydro-6H-pyrido[2,3-b][1,4]benzodiazepin-6-one as ligands for muscarinic receptors.
Novelli F, Sparatore A, Tasso B, Sparatore F.
Bioorg Med Chem Lett (1999) 9 : 3031 -3034
PubMed 10571170 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.39 8.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL9967 PIRENZEPINE 2.0 1 8.42
CHEMBL322378 1 8.10
CHEMBL318316 1 8.00
CHEMBL103189 1 8.00
CHEMBL103785 1 7.55
CHEMBL2111621 1 7.40
CHEMBL2112418 1 6.96
CHEMBL103529 1 6.19
CHEMBL2112432 1 5.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL9967 PIRENZEPINE IC50 = 3.8 nM 8.42
CHEMBL322378 IC50 = 8.0 nM 8.10
CHEMBL318316 IC50 = 10.0 nM 8.00
CHEMBL103189 IC50 = 10.0 nM 8.00
CHEMBL103785 IC50 = 28.0 nM 7.55
CHEMBL2111621 IC50 = 40.0 nM 7.40
CHEMBL2112418 IC50 = 110.0 nM 6.96
CHEMBL103529 IC50 = 650.0 nM 6.19
CHEMBL2112432 IC50 = 1200.0 nM 5.92