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Assay Detail

CHEMBL750147

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of stimulated [3H](+)-MK-801 binding to N-methyl-D-aspartate glutamate receptor in rat cortical membranes incubated with 10 uM glycine.
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Subcellular Membrane
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Glutamate NMDA receptor (CHEMBL1907608)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Synthesis and biological evaluation of a new set of pyrazolo[1,5-c]quinazoline-2-carboxylates as novel excitatory amino acid antagonists.
Varano F, Catarzi D, Colotta V, Filacchioni G, Galli A, Costagli C, Carlà V.
J Med Chem (2002) 45 : 1035 -1044
PubMed 11855983 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 5.16 6.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL274588 1 6.52
CHEMBL8693 1 6.10
CHEMBL9281 1 6.05
CHEMBL9044 1 5.75
CHEMBL275106 1 5.68
CHEMBL266998 1 5.62
CHEMBL276624 1 5.36
CHEMBL267504 1 5.22
CHEMBL267256 1 4.98
CHEMBL427734 1 4.87
CHEMBL9037 1 4.79
CHEMBL9159 1 4.79
CHEMBL8829 1 4.77
CHEMBL8992 1 4.76
CHEMBL267929 1 4.34
CHEMBL267259 1 4.04
CHEMBL268445 1 4.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL274588 IC50 = 300.0 nM 6.52
CHEMBL8693 IC50 = 790.0 nM 6.10
CHEMBL9281 IC50 = 890.0 nM 6.05
CHEMBL9044 IC50 = 1800.0 nM 5.75
CHEMBL275106 IC50 = 2100.0 nM 5.68
CHEMBL266998 IC50 = 2400.0 nM 5.62
CHEMBL276624 IC50 = 4330.0 nM 5.36
CHEMBL267504 IC50 = 6000.0 nM 5.22
CHEMBL267256 IC50 = 10400.0 nM 4.98
CHEMBL427734 IC50 = 13400.0 nM 4.87
CHEMBL9037 IC50 = 16100.0 nM 4.79
CHEMBL9159 IC50 = 16200.0 nM 4.79
CHEMBL8829 IC50 = 17000.0 nM 4.77
CHEMBL8992 IC50 = 17500.0 nM 4.76
CHEMBL267929 IC50 = 46000.0 nM 4.34
CHEMBL267259 IC50 = 92000.0 nM 4.04
CHEMBL268445 IC50 = 91000.0 nM 4.04