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Assay Detail

CHEMBL751320

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of anchorage independent growth of H-ras transformed NIH3T3 cells in Soft Agar
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type NIH3T3
Confidence 1 — Organism
Curated By Expert

Target

NIH3T3 (CHEMBL614822)
Type CELL-LINE
Organism Mus musculus

Publication

Discovery of (R)-7-cyano-2,3,4, 5-tetrahydro-1-(1H-imidazol-4-ylmethyl)-3- (phenylmethyl)-4-(2-thienylsulfonyl)-1H-1,4-benzodiazepine (BMS-214662), a farnesyltransferase inhibitor with potent preclinical antitumor activity.
Hunt JT, Ding CZ, Batorsky R, Bednarz M, Bhide R, Cho Y, Chong S, Chao S, Gullo-Brown J, Guo P, Kim SH, Lee FY, Leftheris K, Miller A, Mitt T, Patel M, Penhallow BA, Ricca C, Rose WC, Schmidt R, Slusarchyk WA, Vite G, Manne V.
J Med Chem (2000) 43 : 3587 -3595
PubMed 11020273 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 7.09 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL331640 1 8.00
CHEMBL118669 1 8.00
CHEMBL118181 1 7.89
CHEMBL330945 1 7.82
CHEMBL351706 BMS-214662 1.0 1 7.60
CHEMBL421235 1 7.52
CHEMBL330815 1 7.40
CHEMBL118616 1 7.30
CHEMBL120683 1 7.00
CHEMBL120071 1 6.57
CHEMBL119771 1 6.54
CHEMBL333251 1 6.52
CHEMBL334073 1 6.40
CHEMBL333589 1 6.34
CHEMBL118526 1 6.28
CHEMBL333288 1 6.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL331640 EC50 = 10.0 nM 8.00
CHEMBL118669 EC50 = 10.0 nM 8.00
CHEMBL118181 EC50 = 13.0 nM 7.89
CHEMBL330945 EC50 = 15.0 nM 7.82
CHEMBL351706 BMS-214662 EC50 = 25.0 nM 7.60
CHEMBL421235 EC50 = 30.0 nM 7.52
CHEMBL330815 EC50 = 40.0 nM 7.40
CHEMBL118616 EC50 = 50.0 nM 7.30
CHEMBL120683 EC50 = 100.0 nM 7.00
CHEMBL120071 EC50 = 270.0 nM 6.57
CHEMBL119771 EC50 = 290.0 nM 6.54
CHEMBL333251 EC50 = 300.0 nM 6.52
CHEMBL334073 EC50 = 400.0 nM 6.40
CHEMBL333589 EC50 = 460.0 nM 6.34
CHEMBL118526 EC50 = 530.0 nM 6.28
CHEMBL333288 EC50 = 600.0 nM 6.22