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Assay Detail

CHEMBL751997

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of purified mouse inducible nitric oxide synthase catalyzed [14C]L-citrulline production at a compound concentration of 1 mM in presence of 50 uM L-arginine
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Nitric oxide synthase, inducible (CHEMBL3464)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Guanidine-substituted imidazoles as inhibitors of nitric oxide synthase.
Atkinson RN, King SB.
Bioorg Med Chem Lett (1999) 9 : 2953 -2958
PubMed 10571154 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.40 4.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL540 IMIDAZOLE 1 4.40
CHEMBL101317 1 -
CHEMBL14189 2-PHENYLIMIDAZOLE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL540 IMIDAZOLE IC50 = 40000.0 nM 4.40
CHEMBL101317 IC50 = 138000.0 nM -
CHEMBL14189 2-PHENYLIMIDAZOLE IC50 > 1000000.0 nM -