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Assay Detail

CHEMBL756787

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of RNA synthesis was measured as the concentration required to cause 50% reduction (in 1h) in 2-[14C]uridine incorporation into acid precipitable material using P388 cells
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type P388
Confidence 1 — Organism
Curated By Intermediate

Target

P388 (CHEMBL389)
Type CELL-LINE
Organism Mus musculus

Publication

"Reverse" and "symmetrical" analogues of actinomycin D: metabolic activation and in vitro and in vivo tumor growth inhibitory activities.
Sengupta SK, Kelly C, Sehgal RK.
J Med Chem (1985) 28 : 620 -628
PubMed 2985784 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 8.97 10.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL439714 1 10.15
CHEMBL406071 1 8.94
CHEMBL267466 1 8.49
CHEMBL385161 1 8.29

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL439714 IC50 = 0.07 nM 10.15
CHEMBL406071 IC50 = 1.15 nM 8.94
CHEMBL267466 IC50 = 3.2 nM 8.49
CHEMBL385161 IC50 = 5.1 nM 8.29