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Assay Detail

CHEMBL757860

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]- naloxone binding to Opioid receptors in rat brain membrane in the absence of Na
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Subcellular Membrane
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Opioid receptor (CHEMBL2094129)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

Synthesis of analogues of acetylmethadol and methadol as potential narcotic antagonists.
Lattin DL, Caviness B, Hudson RG, Greene DL, Raible PK, Richardson JB.
J Med Chem (1981) 24 : 903 -906
PubMed 6268789 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 7.20 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2107793 ALPHACETYLMETHADOL 2.0 1 8.92
CHEMBL159659 LEVOMETHADONE 2.0 1 8.70
CHEMBL2096625 1 8.22
CHEMBL2105619 BETACETYLMETHADOL 2.0 1 7.82
CHEMBL350719 ESMETHADONE 3.0 1 6.96
CHEMBL171118 1 6.82
CHEMBL159660 ALPHAMETHADOL 2.0 1 6.64
CHEMBL355396 1 6.22
CHEMBL1325 1 5.96
CHEMBL367330 1 5.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2107793 ALPHACETYLMETHADOL Ki = 1.2 nM 8.92
CHEMBL159659 LEVOMETHADONE Ki = 2.0 nM 8.70
CHEMBL2096625 Ki = 6.0 nM 8.22
CHEMBL2105619 BETACETYLMETHADOL Ki = 15.0 nM 7.82
CHEMBL350719 ESMETHADONE Ki = 110.0 nM 6.96
CHEMBL171118 Ki = 150.0 nM 6.82
CHEMBL159660 ALPHAMETHADOL Ki = 230.0 nM 6.64
CHEMBL355396 Ki = 600.0 nM 6.22
CHEMBL1325 Ki = 1100.0 nM 5.96
CHEMBL367330 Ki = 2000.0 nM 5.70