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Assay Detail

CHEMBL758225

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Affinity to mu-receptor, using [3H]DAMGO as radioligand in homogenates of guinea pig brain membranes
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Tissue Brain
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Mu-type opioid receptor (CHEMBL4354)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Methylated analogues of methyl (R)-4-(3,4-dichlorophenylacetyl)- 3-(pyrrolidin-1-ylmethyl)piperazine-1-carboxylate (GR-89,696) as highly potent kappa-receptor agonists: stereoselective synthesis, opioid-receptor affinity, receptor selectivity, and functional studies.
Soukara S, Maier CA, Predoiu U, Ehret A, Jackisch R, Wünsch B.
J Med Chem (2001) 44 : 2814 -2826
PubMed 11495592 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 7.88 9.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1094879 1 9.44
CHEMBL80 NALOXONE 4.0 1 9.17
CHEMBL1095519 1 8.17
CHEMBL2114291 1 4.72
CHEMBL2114294 1 -
CHEMBL94320 1 -
CHEMBL2114292 1 -
CHEMBL2114296 1 -
CHEMBL2115272 1 -
CHEMBL2114293 1 -
CHEMBL97051 1 -
CHEMBL97590 1 -
CHEMBL2115271 1 -
CHEMBL2115270 1 -
CHEMBL319835 1 -
CHEMBL2114295 1 -
CHEMBL2115273 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1094879 Ki = 0.36 nM 9.44
CHEMBL80 NALOXONE Ki = 0.68 nM 9.17
CHEMBL1095519 Ki = 6.8 nM 8.17
CHEMBL2114291 Ki = 19100.0 nM 4.72
CHEMBL2114294 Ki > 10000.0 nM -
CHEMBL94320 Ki > 10000.0 nM -
CHEMBL2114292 Ki > 1000.0 nM -
CHEMBL2114296 Ki > 1000.0 nM -
CHEMBL2115272 Ki > 10000.0 nM -
CHEMBL2114293 Ki > 1000.0 nM -
CHEMBL97051 Ki > 1000.0 nM -
CHEMBL97590 Ki > 1000.0 nM -
CHEMBL2115271 Ki > 10000.0 nM -
CHEMBL2115270 Ki > 10000.0 nM -
CHEMBL319835 Ki > 1000.0 nM -
CHEMBL2114295 Ki > 1000.0 nM -
CHEMBL2115273 Ki > 1000.0 nM -