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Assay Detail

CHEMBL765478

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human Phosphodiesterase 4A isoform using construct representing the common region of spliced variants expressed as GST-fusion proteins in Sf9 cells.
18
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type Sf9
Confidence 8 — Homologous single protein target
Curated By Expert

Target

3',5'-cyclic-AMP phosphodiesterase 4A (CHEMBL254)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Substituted 4-(2,2-diphenylethyl)pyridine-N-oxides as phosphodiesterase-4 inhibitors: SAR study directed toward the improvement of pharmacokinetic parameters.
Frenette R, Blouin M, Brideau C, Chauret N, Ducharme Y, Friesen RW, Hamel P, Jones TR, Laliberté F, Li C, Masson P, McAuliffe M, Girard Y.
Bioorg Med Chem Lett (2002) 12 : 3009 -3013
PubMed 12270195 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.95 8.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL323312 5 8.89
CHEMBL408345 1 8.57
CHEMBL286224 1 8.38
CHEMBL32442 CDP840 1 8.38
CHEMBL103635 1 8.36
CHEMBL105164 1 7.75
CHEMBL103859 1 7.57
CHEMBL102718 1 7.50
CHEMBL511115 CILOMILAST 3.0 1 7.42
CHEMBL105243 1 7.42
CHEMBL102784 2 7.41
CHEMBL319858 1 7.38
CHEMBL318108 1 7.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL323312 IC50 = 1.3 nM 8.89
CHEMBL323312 IC50 = 2.1 nM 8.68
CHEMBL323312 IC50 = 2.1 nM 8.68
CHEMBL408345 IC50 = 2.7 nM 8.57
CHEMBL32442 CDP840 IC50 = 4.2 nM 8.38
CHEMBL323312 IC50 = 4.2 nM 8.38
CHEMBL286224 IC50 = 4.2 nM 8.38
CHEMBL103635 IC50 = 4.4 nM 8.36
CHEMBL105164 IC50 = 18.0 nM 7.75
CHEMBL323312 IC50 = 23.0 nM 7.64
CHEMBL103859 IC50 = 27.0 nM 7.57
CHEMBL102718 IC50 = 32.0 nM 7.50
CHEMBL105243 IC50 = 38.0 nM 7.42
CHEMBL511115 CILOMILAST IC50 = 38.0 nM 7.42
CHEMBL102784 IC50 = 39.0 nM 7.41
CHEMBL102784 IC50 = 39.0 nM 7.41
CHEMBL319858 IC50 = 42.0 nM 7.38
CHEMBL318108 IC50 = 57.0 nM 7.24