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Assay Detail

CHEMBL769148

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro for inhibitory activity against myosin light-chain kinase from chicken gizzard (M-kinase)
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Myosin light chain kinase, smooth muscle (CHEMBL3062)
Type SINGLE PROTEIN
Organism Gallus gallus

Publication

Non-glycosidic/non-aminoalkyl-substituted indolocarbazoles as inhibitors of protein kinase C
Kleinschroth J, Hartenstein J, Rudolph C, Schachtele C
Bioorg Med Chem Lett (1993) 3 : 1959 -1964
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.07 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL388978 STAUROSPORINE 1 8.00
CHEMBL59384 1 7.09
CHEMBL291920 1 6.41
CHEMBL303990 1 6.36
CHEMBL59385 1 6.35
CHEMBL292746 1 6.30
CHEMBL59004 1 5.96
CHEMBL301228 1 5.92
CHEMBL58458 1 5.80
CHEMBL59009 1 5.75
CHEMBL16958 STAUROSPORINONE 1 5.55
CHEMBL301620 1 5.24
CHEMBL302449 GO-6976 1 5.24
CHEMBL59054 1 4.96
CHEMBL61753 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL388978 STAUROSPORINE IC50 = 10.0 nM 8.00
CHEMBL59384 IC50 = 82.0 nM 7.09
CHEMBL291920 IC50 = 390.0 nM 6.41
CHEMBL303990 IC50 = 440.0 nM 6.36
CHEMBL59385 IC50 = 450.0 nM 6.35
CHEMBL292746 IC50 = 500.0 nM 6.30
CHEMBL59004 IC50 = 1100.0 nM 5.96
CHEMBL301228 IC50 = 1200.0 nM 5.92
CHEMBL58458 IC50 = 1600.0 nM 5.80
CHEMBL59009 IC50 = 1800.0 nM 5.75
CHEMBL16958 STAUROSPORINONE IC50 = 2800.0 nM 5.55
CHEMBL301620 IC50 = 5800.0 nM 5.24
CHEMBL302449 GO-6976 IC50 = 5800.0 nM 5.24
CHEMBL59054 IC50 = 11000.0 nM 4.96
CHEMBL61753 IC50 > 10000.0 nM -