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Assay Detail

CHEMBL795061

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of purified recombinant human Ribonuclease L by the compound was measured as degradation of [32P]-pC11U2C7
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

2-5A-dependent ribonuclease (CHEMBL3575)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

2-Methyladenosine-Substituted 2',5'-oligoadenylates: conformations, 2-5A binding and catalytic activities with human ribonuclease L.
Kitade Y, Wakana M, Tsuboi T, Yatome C, Bayly SF, Player MR, Torrence PF.
Bioorg Med Chem Lett (2000) 10 : 329 -331
PubMed 10714492 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 7 9.01 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL414948 1 9.70
CHEMBL402676 1 9.70
CHEMBL214603 1 9.52
CHEMBL384725 1 9.15
CHEMBL405496 1 9.10
CHEMBL410213 1 8.40
CHEMBL407239 1 7.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL414948 EC50 = 0.2 nM 9.70
CHEMBL402676 EC50 = 0.2 nM 9.70
CHEMBL214603 EC50 = 0.3 nM 9.52
CHEMBL384725 EC50 = 0.7 nM 9.15
CHEMBL405496 EC50 = 0.8 nM 9.10
CHEMBL410213 EC50 = 4.0 nM 8.40
CHEMBL407239 EC50 = 30.0 nM 7.52