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Assay Detail

CHEMBL803934

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Sigma receptor binding was determined by labeling with radioligand [3H](+)-pentazocine on frozen membranes obtained from frozen guinea pig brain
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Sigma non-opioid intracellular receptor 1 (CHEMBL287)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Heteroaromatic analogs of 1-[2-(diphenylmethoxy)ethyl]- and 1-[2-[bis(4-fluorophenyl)methoxy]ethyl]-4-(3-phenylpropyl)piperazines (GBR 12935 and GBR 12909) as high-affinity dopamine reuptake inhibitors.
Matecka D, Lewis D, Rothman RB, Dersch CM, Wojnicki FH, Glowa JR, DeVries AC, Pert A, Rice KC.
J Med Chem (1997) 40 : 705 -716
PubMed 9057857 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.35 7.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL26320 1 7.77
CHEMBL163980 1 7.42
CHEMBL159320 1 7.39
CHEMBL159967 1 7.37
CHEMBL281594 VANOXERINE 3.0 1 7.37
CHEMBL286991 1 7.29
CHEMBL164037 1 7.23
CHEMBL159319 1 7.21
CHEMBL159608 1 7.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL26320 IC50 = 17.0 nM 7.77
CHEMBL163980 IC50 = 38.0 nM 7.42
CHEMBL159320 IC50 = 41.0 nM 7.39
CHEMBL159967 IC50 = 43.0 nM 7.37
CHEMBL281594 VANOXERINE IC50 = 43.0 nM 7.37
CHEMBL286991 IC50 = 51.0 nM 7.29
CHEMBL164037 IC50 = 59.0 nM 7.23
CHEMBL159319 IC50 = 62.0 nM 7.21
CHEMBL159608 IC50 = 79.0 nM 7.10