Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL821236

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Compound was evaluated for the inhibition of dTMP synthetase with respect to dUMP.
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Thymidylate synthase (CHEMBL6137)
Type SINGLE PROTEIN
Organism Escherichia coli

Publication

Interaction of 1-(5-phospho-beta-D-arabinofuranosyl)-5-substituted-uracils with thymidylate synthetase: mechanism-based inhibition by 1-(5-phospho-beta-D-arabinosyl)-5-fluorouracil.
Nakayama C, Wataya Y, Santi DV, Saneyoshi M, Ueda T.
J Med Chem (1981) 24 : 1161 -1165
PubMed 7328576 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 5.98 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL886 5-FLUORODEOXYURIDYLATE 1 8.00
CHEMBL603555 1 6.60
CHEMBL235506 1 4.80
CHEMBL603336 1 4.52
CHEMBL606019 1 -
CHEMBL2311177 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL886 5-FLUORODEOXYURIDYLATE Ki = 10.0 nM 8.00
CHEMBL603555 Ki = 250.0 nM 6.60
CHEMBL235506 Ki = 16000.0 nM 4.80
CHEMBL603336 Ki = 30000.0 nM 4.52
CHEMBL606019 Ki = 230000.0 nM -
CHEMBL2311177 Ki = 120000.0 nM -