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Assay Detail

CHEMBL822946

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Potentiation of GABA-activated currents in Xenopus oocytes expressing cloned human alpha-1-beta-2-gamma-2L subunits of GABA-A receptor
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Oocyte
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GABA-A receptor; alpha-1/beta-2/gamma-2 (CHEMBL2095172)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Synthesis and in vitro activity of 3 beta-substituted-3 alpha-hydroxypregnan-20-ones: allosteric modulators of the GABAA receptor.
Hogenkamp DJ, Tahir SH, Hawkinson JE, Upasani RB, Alauddin M, Kimbrough CL, Acosta-Burruel M, Whittemore ER, Woodward RM, Lan NC, Gee KW, Bolger MB.
J Med Chem (1997) 40 : 61 -72
PubMed 9016329 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 6.65 6.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3137994 1 6.82
CHEMBL207538 BREXANOLONE 4.0 1 6.80
CHEMBL1568698 GANAXOLONE 4.0 1 6.58
CHEMBL3137990 1 6.41
CHEMBL3137988 1 -
CHEMBL3137984 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3137994 EC50 = 150.0 nM 6.82
CHEMBL207538 BREXANOLONE EC50 = 160.0 nM 6.80
CHEMBL1568698 GANAXOLONE EC50 = 260.0 nM 6.58
CHEMBL3137990 EC50 = 390.0 nM 6.41
CHEMBL3137988 EC50 ~ 20000.0 nM -
CHEMBL3137984 EC50 > 50000.0 nM -