Assay Detail
Functional
CHEMBL863412
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Activity against 5HT2A mediated intracellular calcium mobilization by FLIPR in rat vascular smooth muscle cells
26
Total Activities
13
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Rattus norvegicus |
| Confidence | 9 — Direct single protein target |
| Curated By | Expert |
Publication
1-((S)-2-aminopropyl)-1H-indazol-6-ol: a potent peripherally acting 5-HT2 receptor agonist with ocular hypotensive activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 13 | 7.11 | 7.70 |
| Emax | 13 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL134519 | — | — | 2 | 7.70 |
| CHEMBL371300 | — | — | 2 | 7.37 |
| CHEMBL31115 | — | — | 2 | 7.32 |
| CHEMBL199775 | — | — | 2 | 7.27 |
| CHEMBL39 | SEROTONIN | 3.0 | 2 | 7.24 |
| CHEMBL275854 | — | — | 2 | 7.24 |
| CHEMBL197653 | — | — | 2 | 7.24 |
| CHEMBL373245 | — | — | 2 | 7.22 |
| CHEMBL199385 | — | — | 2 | 7.13 |
| CHEMBL200104 | — | — | 2 | 7.09 |
| CHEMBL201132 | — | — | 2 | 6.69 |
| CHEMBL437314 | — | — | 2 | 6.63 |
| CHEMBL7257 | MEBUFOTENIN | 2.0 | 2 | 6.33 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL134519 | — | EC50 | = | 19.8 | nM | 7.70 |
| CHEMBL371300 | — | EC50 | = | 42.7 | nM | 7.37 |
| CHEMBL31115 | — | EC50 | = | 47.5 | nM | 7.32 |
| CHEMBL199775 | — | EC50 | = | 54.0 | nM | 7.27 |
| CHEMBL275854 | — | EC50 | = | 58.0 | nM | 7.24 |
| CHEMBL39 | SEROTONIN | EC50 | = | 57.9 | nM | 7.24 |
| CHEMBL197653 | — | EC50 | = | 58.1 | nM | 7.24 |
| CHEMBL373245 | — | EC50 | = | 59.7 | nM | 7.22 |
| CHEMBL199385 | — | EC50 | = | 74.4 | nM | 7.13 |
| CHEMBL200104 | — | EC50 | = | 81.3 | nM | 7.09 |
| CHEMBL201132 | — | EC50 | = | 204.0 | nM | 6.69 |
| CHEMBL437314 | — | EC50 | = | 236.0 | nM | 6.63 |
| CHEMBL7257 | MEBUFOTENIN | EC50 | = | 462.0 | nM | 6.33 |
| CHEMBL371300 | — | Emax | = | 81.0 | % | - |
| CHEMBL134519 | — | Emax | = | 35.0 | % | - |
| CHEMBL199385 | — | Emax | = | 75.0 | % | - |
| CHEMBL275854 | — | Emax | = | 96.0 | % | - |
| CHEMBL39 | SEROTONIN | Emax | = | 99.0 | % | - |
| CHEMBL437314 | — | Emax | = | 61.0 | % | - |
| CHEMBL199775 | — | Emax | = | 90.0 | % | - |
| CHEMBL7257 | MEBUFOTENIN | Emax | = | 70.0 | % | - |
| CHEMBL201132 | — | Emax | = | 73.0 | % | - |
| CHEMBL373245 | — | Emax | = | 87.0 | % | - |
| CHEMBL197653 | — | Emax | = | 80.0 | % | - |
| CHEMBL31115 | — | Emax | = | 96.0 | % | - |
| CHEMBL200104 | — | Emax | = | 89.0 | % | - |