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Assay Detail

CHEMBL868533

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of dog bronchi prototypical epithelial sodium channel by electrophysiological assay
42
Total Activities
42
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Canis lupus familiaris
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Amiloride-sensitive sodium channel subunit alpha (CHEMBL1791)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and structure-activity relationships of novel 2-substituted pyrazinoylguanidine epithelial sodium channel blockers: drugs for cystic fibrosis and chronic bronchitis.
Hirsh AJ, Molino BF, Zhang J, Astakhova N, Geiss WB, Sargent BJ, Swenson BD, Usyatinsky A, Wyle MJ, Boucher RC, Smith RT, Zamurs A, Johnson MR.
J Med Chem (2006) 49 : 4098 -4115
PubMed 16821771 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 42 7.40 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL212431 1 8.15
CHEMBL212531 1 8.15
CHEMBL212432 552-02 FREE BASE 2.0 1 8.10
CHEMBL209303 1 8.05
CHEMBL211161 1 8.05
CHEMBL214239 1 7.85
CHEMBL377986 1 7.82
CHEMBL211600 1 7.77
CHEMBL210876 1 7.77
CHEMBL380011 1 7.70
CHEMBL379433 1 7.70
CHEMBL212410 1 7.68
CHEMBL386222 1 7.68
CHEMBL379756 1 7.66
CHEMBL214220 1 7.60
CHEMBL212534 1 7.58
CHEMBL211658 1 7.55
CHEMBL209659 1 7.54
CHEMBL379066 1 7.52
CHEMBL208889 1 7.51
CHEMBL211946 1 7.50
CHEMBL211773 1 7.39
CHEMBL211072 1 7.38
CHEMBL209017 1 7.32
CHEMBL211664 1 7.30
CHEMBL213175 1 7.29
CHEMBL208620 1 7.24
CHEMBL209977 1 7.21
CHEMBL211221 1 7.19
CHEMBL212579 BENZAMIL 1 7.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL212431 IC50 = 7.0 nM 8.15
CHEMBL212531 IC50 = 7.0 nM 8.15
CHEMBL212432 552-02 FREE BASE IC50 = 8.0 nM 8.10
CHEMBL209303 IC50 = 9.0 nM 8.05
CHEMBL211161 IC50 = 9.0 nM 8.05
CHEMBL214239 IC50 = 14.0 nM 7.85
CHEMBL377986 IC50 = 15.0 nM 7.82
CHEMBL211600 IC50 = 17.0 nM 7.77
CHEMBL210876 IC50 = 17.0 nM 7.77
CHEMBL380011 IC50 = 20.0 nM 7.70
CHEMBL379433 IC50 = 20.0 nM 7.70
CHEMBL386222 IC50 = 21.0 nM 7.68
CHEMBL212410 IC50 = 21.0 nM 7.68
CHEMBL379756 IC50 = 22.0 nM 7.66
CHEMBL214220 IC50 = 25.0 nM 7.60
CHEMBL212534 IC50 = 26.0 nM 7.58
CHEMBL211658 IC50 = 28.0 nM 7.55
CHEMBL209659 IC50 = 29.0 nM 7.54
CHEMBL379066 IC50 = 30.0 nM 7.52
CHEMBL208889 IC50 = 31.0 nM 7.51
CHEMBL211946 IC50 = 32.0 nM 7.50
CHEMBL211773 IC50 = 41.0 nM 7.39
CHEMBL211072 IC50 = 42.0 nM 7.38
CHEMBL209017 IC50 = 48.0 nM 7.32
CHEMBL211664 IC50 = 50.0 nM 7.30
CHEMBL213175 IC50 = 51.0 nM 7.29
CHEMBL208620 IC50 = 58.0 nM 7.24
CHEMBL209977 IC50 = 62.0 nM 7.21
CHEMBL211221 IC50 = 64.0 nM 7.19
CHEMBL212579 BENZAMIL IC50 = 66.0 nM 7.18
CHEMBL211611 IC50 = 66.0 nM 7.18
CHEMBL377239 IC50 = 68.0 nM 7.17
CHEMBL380393 IC50 = 73.0 nM 7.14
CHEMBL209104 IC50 = 77.0 nM 7.11
CHEMBL211793 IC50 = 88.0 nM 7.06
CHEMBL210966 IC50 = 87.0 nM 7.06
CHEMBL377688 IC50 = 107.0 nM 6.97
CHEMBL210981 IC50 = 108.0 nM 6.97
CHEMBL210967 IC50 = 124.0 nM 6.91
CHEMBL211502 PHENAMIL IC50 = 401.0 nM 6.40
CHEMBL210866 IC50 = 477.0 nM 6.32
CHEMBL945 AMILORIDE IC50 = 776.0 nM 6.11