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Assay Detail

CHEMBL877809

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Ability to inhibit [125I]ET1 binding to vascular smooth muscle (vsm)- A10 cells
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Oryctolagus cuniculus
Cell Type A10
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Endothelin-1 receptor (CHEMBL252)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The discovery of sulfonamide endothelin antagonists and the development of the orally active ETA antagonist 5-(dimethylamino)-N-(3,4-dimethyl-5-isoxazolyl)-1-naphthalenesulf onamide.
Stein PD, Hunt JT, Floyd DM, Moreland S, Dickinson KE, Mitchell C, Liu EC, Webb ML, Murugesan N, Dickey J.
J Med Chem (1994) 37 : 329 -331
PubMed 8308857 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.23 6.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL267458 BMS-182874 1 6.82
CHEMBL453 SULFISOXAZOLE 4.0 1 6.11
CHEMBL30199 1 6.06
CHEMBL27525 1 5.55
CHEMBL30039 1 5.40
CHEMBL281942 1 5.24
CHEMBL28692 1 5.19
CHEMBL282961 1 5.04
CHEMBL25730 1 5.01
CHEMBL30246 1 4.89
CHEMBL27065 1 4.70
CHEMBL286682 1 4.57
CHEMBL283941 1 4.55
CHEMBL332471 1 4.08
CHEMBL325690 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL267458 BMS-182874 IC50 = 150.0 nM 6.82
CHEMBL453 SULFISOXAZOLE IC50 = 780.0 nM 6.11
CHEMBL30199 IC50 = 880.0 nM 6.06
CHEMBL27525 IC50 = 2800.0 nM 5.55
CHEMBL30039 IC50 = 4000.0 nM 5.40
CHEMBL281942 IC50 = 5700.0 nM 5.24
CHEMBL28692 IC50 = 6500.0 nM 5.19
CHEMBL282961 IC50 = 9200.0 nM 5.04
CHEMBL25730 IC50 = 9800.0 nM 5.01
CHEMBL30246 IC50 = 13000.0 nM 4.89
CHEMBL27065 IC50 = 20000.0 nM 4.70
CHEMBL286682 IC50 = 27000.0 nM 4.57
CHEMBL283941 IC50 = 28000.0 nM 4.55
CHEMBL332471 IC50 = 84000.0 nM 4.08
CHEMBL325690 IC50 > 32000.0 nM -