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Assay Detail

CHEMBL887397

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human mu opioid receptor expressed in CHO cells assessed as DAMGO-stimulated [35S]GTP-gamma-S binding
24
Total Activities
12
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Expert

Target

Mu-type opioid receptor (CHEMBL233)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

High-affinity carbamate analogues of morphinan at opioid receptors.
Peng X, Knapp BI, Bidlack JM, Neumeyer JL.
Bioorg Med Chem Lett (2007) 17 : 1508 -1511
PubMed 17276685 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Imax 12 - -
IC50 11 7.81 8.77
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL49269 CYCLORPHAN 2 8.77
CHEMBL397035 2 8.19
CHEMBL427862 2 8.00
CHEMBL263096 2 7.80
CHEMBL301160 BUTORPHAN 2 7.70
CHEMBL242048 2 7.66
CHEMBL394624 2 6.58
CHEMBL38874 DAMGO 2 -
CHEMBL242047 2 -
CHEMBL242046 2 -
CHEMBL241824 2 -
CHEMBL245049 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL49269 CYCLORPHAN IC50 = 1.7 nM 8.77
CHEMBL397035 IC50 = 6.4 nM 8.19
CHEMBL427862 IC50 = 10.0 nM 8.00
CHEMBL263096 IC50 = 16.0 nM 7.80
CHEMBL301160 BUTORPHAN IC50 = 20.0 nM 7.70
CHEMBL242048 IC50 = 22.0 nM 7.66
CHEMBL394624 IC50 = 260.0 nM 6.58
CHEMBL38874 DAMGO IC50 - -
CHEMBL394624 Imax = 50.0 % -
CHEMBL242048 Imax = 37.0 % -
CHEMBL242047 Imax = 33.0 % -
CHEMBL242047 Activity - -
CHEMBL242046 Imax - -
CHEMBL242046 IC50 - -
CHEMBL301160 BUTORPHAN Imax = 50.0 % -
CHEMBL38874 DAMGO Imax - -
CHEMBL397035 Imax = 70.0 % -
CHEMBL427862 Imax = 68.0 % -
CHEMBL241824 Imax - -
CHEMBL241824 IC50 - -
CHEMBL49269 CYCLORPHAN Imax = 50.0 % -
CHEMBL245049 Imax - -
CHEMBL245049 IC50 - -
CHEMBL263096 Imax = 71.0 % -