Assay Detail
Functional
CHEMBL899150
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at muscarinic M3 receptor in guinea-pig ileum
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Cavia porcellus |
| Tissue | Ileum |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M3 (CHEMBL5498) ↗| Type | SINGLE PROTEIN |
| Organism | Cavia porcellus |
Publication
Novel oxotremorine-related heterocyclic derivatives: Synthesis and in vitro pharmacology at the muscarinic receptor subtypes.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 9 | 6.16 | 8.14 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL239655 | — | — | 1 | 8.14 |
| CHEMBL239436 | — | — | 1 | 6.57 |
| CHEMBL1482 | BETHANECHOL | 4.0 | 1 | 6.37 |
| CHEMBL238825 | — | — | 1 | 6.26 |
| CHEMBL239017 | — | — | 1 | 6.10 |
| CHEMBL240763 | — | — | 1 | 5.99 |
| CHEMBL239866 | — | — | 1 | 5.75 |
| CHEMBL240764 | — | — | 1 | 5.45 |
| CHEMBL397083 | — | — | 1 | 4.82 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL239655 | — | EC50 | = | 7.244 | nM | 8.14 |
| CHEMBL239436 | — | EC50 | = | 269.15 | nM | 6.57 |
| CHEMBL1482 | BETHANECHOL | EC50 | = | 426.58 | nM | 6.37 |
| CHEMBL238825 | — | EC50 | = | 549.54 | nM | 6.26 |
| CHEMBL239017 | — | EC50 | = | 794.33 | nM | 6.10 |
| CHEMBL240763 | — | EC50 | = | 1023.29 | nM | 5.99 |
| CHEMBL239866 | — | EC50 | = | 1778.28 | nM | 5.75 |
| CHEMBL240764 | — | EC50 | = | 3548.13 | nM | 5.45 |
| CHEMBL397083 | — | EC50 | = | 15135.61 | nM | 4.82 |