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Assay Detail

CHEMBL917978

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human cloned muscarinic receptor M2 expressed in CHO cells
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M2 (CHEMBL211)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis, affinity profile, and functional activity of muscarinic antagonists with a 1-methyl-2-(2,2-alkylaryl-1,3-oxathiolan-5-yl)pyrrolidine structure.
Dei S, Bellucci C, Buccioni M, Ferraroni M, Guandalini L, Manetti D, Martini E, Marucci G, Matucci R, Nesi M, Romanelli MN, Scapecchi S, Teodori E.
J Med Chem (2007) 50 : 1409 -1413
PubMed 17305327 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 8.77 9.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL376897 METHSCOPOLAMINE 4.0 1 9.75
CHEMBL375969 1 9.33
CHEMBL376057 1 9.11
CHEMBL222259 1 9.08
CHEMBL219786 1 9.02
CHEMBL376870 1 8.97
CHEMBL220393 1 8.83
CHEMBL385328 1 8.56
CHEMBL375073 1 8.56
CHEMBL375851 1 8.47
CHEMBL376255 1 8.37
CHEMBL219825 1 8.35
CHEMBL373515 1 8.31
CHEMBL45454 1 8.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL376897 METHSCOPOLAMINE Ki = 0.1778 nM 9.75
CHEMBL375969 Ki = 0.4677 nM 9.33
CHEMBL376057 Ki = 0.7762 nM 9.11
CHEMBL222259 Ki = 0.8318 nM 9.08
CHEMBL219786 Ki = 0.955 nM 9.02
CHEMBL376870 Ki = 1.072 nM 8.97
CHEMBL220393 Ki = 1.479 nM 8.83
CHEMBL385328 Ki = 2.754 nM 8.56
CHEMBL375073 Ki = 2.754 nM 8.56
CHEMBL375851 Ki = 3.388 nM 8.47
CHEMBL376255 Ki = 4.266 nM 8.37
CHEMBL219825 Ki = 4.467 nM 8.35
CHEMBL373515 Ki = 4.898 nM 8.31
CHEMBL45454 Ki = 7.586 nM 8.12