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Assay Detail

CHEMBL929225

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]5-HT uptake at human recombinant 5HT transporter expressed in HEK293 cells
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent serotonin transporter (CHEMBL228)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pyridyl-phenyl ether monoamine reuptake inhibitors: Impact of lipophilicity on dual SNRI pharmacology and off-target promiscuity.
Whitlock GA, Fish PV, Fray MJ, Stobie A, Wakenhut F.
Bioorg Med Chem Lett (2008) 18 : 2896 -2899
PubMed 18417343 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.89 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL401596 1 8.52
CHEMBL403078 1 8.40
CHEMBL256136 1 8.40
CHEMBL257593 1 8.22
CHEMBL256137 1 8.22
CHEMBL256970 1 8.22
CHEMBL402345 1 8.15
CHEMBL260036 1 8.10
CHEMBL247733 1 8.05
CHEMBL402371 1 8.00
CHEMBL402000 1 7.96
CHEMBL403080 1 7.89
CHEMBL256511 1 7.85
CHEMBL258449 1 7.85
CHEMBL403441 1 7.85
CHEMBL260038 1 7.75
CHEMBL256293 1 7.72
CHEMBL403251 1 7.70
CHEMBL256135 1 7.64
CHEMBL260037 1 7.62
CHEMBL259136 1 7.41
CHEMBL257592 1 7.34
CHEMBL260039 1 6.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL401596 IC50 = 3.0 nM 8.52
CHEMBL403078 IC50 = 4.0 nM 8.40
CHEMBL256136 IC50 = 4.0 nM 8.40
CHEMBL257593 IC50 = 6.0 nM 8.22
CHEMBL256137 IC50 = 6.0 nM 8.22
CHEMBL256970 IC50 = 6.0 nM 8.22
CHEMBL402345 IC50 = 7.0 nM 8.15
CHEMBL260036 IC50 = 8.0 nM 8.10
CHEMBL247733 IC50 = 9.0 nM 8.05
CHEMBL402371 IC50 = 10.0 nM 8.00
CHEMBL402000 IC50 = 11.0 nM 7.96
CHEMBL403080 IC50 = 13.0 nM 7.89
CHEMBL256511 IC50 = 14.0 nM 7.85
CHEMBL258449 IC50 = 14.0 nM 7.85
CHEMBL403441 IC50 = 14.0 nM 7.85
CHEMBL260038 IC50 = 18.0 nM 7.75
CHEMBL256293 IC50 = 19.0 nM 7.72
CHEMBL403251 IC50 = 20.0 nM 7.70
CHEMBL256135 IC50 = 23.0 nM 7.64
CHEMBL260037 IC50 = 24.0 nM 7.62
CHEMBL259136 IC50 = 39.0 nM 7.41
CHEMBL257592 IC50 = 46.0 nM 7.34
CHEMBL260039 IC50 = 231.0 nM 6.64