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Assay Detail

CHEMBL941014

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Mitotic arrest in human A2780 cells assessed as G2/M block after 16 hrs
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type A2780
Confidence 1 — Organism
Curated By Autocuration

Target

A2780 (CHEMBL614004)
Type CELL-LINE
Organism Homo sapiens

Publication

Kinesin spindle protein (KSP) inhibitors. Part 7: Design and synthesis of 3,3-disubstituted dihydropyrazolobenzoxazines as potent inhibitors of the mitotic kinesin KSP.
Garbaccio RM, Tasber ES, Neilson LA, Coleman PJ, Fraley ME, Olson C, Bergman J, Torrent M, Buser CA, Rickert K, Walsh ES, Hamilton K, Lobell RB, Tao W, South VJ, Diehl RE, Davide JP, Yan Y, Kuo LC, Li C, Prueksaritanont T, Fernandez-Metzler C, Mahan EA, Slaughter DE, Salata JJ, Kohl NE, Huber HE, Hartman GD.
Bioorg Med Chem Lett (2007) 17 : 5671 -5676
PubMed 17804233 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 8.20 8.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL250127 1 8.60
CHEMBL398478 1 8.36
CHEMBL251142 1 8.32
CHEMBL429553 1 8.32
CHEMBL401476 1 8.30
CHEMBL250125 1 8.30
CHEMBL250532 1 8.19
CHEMBL438876 1 8.16
CHEMBL250328 1 8.13
CHEMBL250126 1 8.05
CHEMBL250743 1 7.96
CHEMBL399378 1 7.96
CHEMBL250533 1 7.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL250127 EC50 = 2.5 nM 8.60
CHEMBL398478 EC50 = 4.4 nM 8.36
CHEMBL251142 EC50 = 4.8 nM 8.32
CHEMBL429553 EC50 = 4.8 nM 8.32
CHEMBL401476 EC50 = 5.0 nM 8.30
CHEMBL250125 EC50 = 5.0 nM 8.30
CHEMBL250532 EC50 = 6.4 nM 8.19
CHEMBL438876 EC50 = 6.9 nM 8.16
CHEMBL250328 EC50 = 7.4 nM 8.13
CHEMBL250126 EC50 = 9.0 nM 8.05
CHEMBL250743 EC50 = 11.0 nM 7.96
CHEMBL399378 EC50 = 11.0 nM 7.96
CHEMBL250533 EC50 = 13.0 nM 7.89