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Assay Detail

CHEMBL955982

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant Pim1 expressed in insect cells by HTRF
12
Total Activities
12
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase pim-1 (CHEMBL2147)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Indolyl-pyrrolone as a new scaffold for Pim1 inhibitors.
Olla S, Manetti F, Crespan E, Maga G, Angelucci A, Schenone S, Bologna M, Botta M.
Bioorg Med Chem Lett (2009) 19 : 1512 -1516
PubMed 19179076 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 7 5.52 5.68
IC50 5 6.14 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL388978 STAUROSPORINE 1 8.00
CHEMBL268769 1 6.82
CHEMBL478510 1 5.68
CHEMBL476454 1 5.68
CHEMBL478324 1 5.66
CHEMBL476452 1 5.40
CHEMBL478323 1 5.38
CHEMBL476025 1 5.35
CHEMBL515778 1 4.80
CHEMBL515495 1 -
CHEMBL478141 1 -
CHEMBL500812 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL388978 STAUROSPORINE IC50 = 10.0 nM 8.00
CHEMBL268769 IC50 = 150.0 nM 6.82
CHEMBL478510 Ki = 2100.0 nM 5.68
CHEMBL476454 IC50 = 2100.0 nM 5.68
CHEMBL478324 Ki = 2200.0 nM 5.66
CHEMBL476452 IC50 = 4000.0 nM 5.40
CHEMBL478323 Ki = 4200.0 nM 5.38
CHEMBL476025 Ki = 4500.0 nM 5.35
CHEMBL515778 IC50 = 16000.0 nM 4.80
CHEMBL515495 Ki - -
CHEMBL478141 Ki - -
CHEMBL500812 Ki - -