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Assay Detail

CHEMBL960988

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against human HCT116 cells after 72 hrs by luminescence-based cell viability assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HCT-116
Confidence 1 — Organism
Curated By Autocuration

Target

HCT-116 (CHEMBL394)
Type CELL-LINE
Organism Homo sapiens

Publication

Design of potent thiophene inhibitors of polo-like kinase 1 with improved solubility and reduced protein binding.
Emmitte KA, Adjabeng GM, Andrews CW, Alberti JG, Bambal R, Chamberlain SD, Davis-Ward RG, Dickson HD, Hassler DF, Hornberger KR, Jackson JR, Kuntz KW, Lansing TJ, Mook RA, Nailor KE, Pobanz MA, Smith SC, Sung CM, Cheung M.
Bioorg Med Chem Lett (2009) 19 : 1694 -1697
PubMed 19237286 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.25 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1794070 GSK-579289A 1 7.96
CHEMBL1615278 1 7.92
CHEMBL1964265 1 7.89
CHEMBL1964263 1 7.82
CHEMBL1964264 1 7.77
CHEMBL222419 GSK-237701A 1 7.60
CHEMBL1794068 GSK-326090A 1 7.52
CHEMBL1964261 1 7.50
CHEMBL1794069 GSK-571989A 1 7.47
CHEMBL1964262 1 7.35
CHEMBL1794067 GSK-978744A 1 7.14
CHEMBL493326 GSK-317314A 1 6.49
CHEMBL522709 GSK-317315A 1 6.32
CHEMBL521806 GSK-237700A 1 6.00
CHEMBL514162 GW852849X 1 5.94

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1794070 GSK-579289A IC50 = 11.0 nM 7.96
CHEMBL1615278 IC50 = 12.0 nM 7.92
CHEMBL1964265 IC50 = 13.0 nM 7.89
CHEMBL1964263 IC50 = 15.0 nM 7.82
CHEMBL1964264 IC50 = 17.0 nM 7.77
CHEMBL222419 GSK-237701A IC50 = 25.0 nM 7.60
CHEMBL1794068 GSK-326090A IC50 = 30.0 nM 7.52
CHEMBL1964261 IC50 = 32.0 nM 7.50
CHEMBL1794069 GSK-571989A IC50 = 34.0 nM 7.47
CHEMBL1964262 IC50 = 45.0 nM 7.35
CHEMBL1794067 GSK-978744A IC50 = 73.0 nM 7.14
CHEMBL493326 GSK-317314A IC50 = 321.0 nM 6.49
CHEMBL522709 GSK-317315A IC50 = 476.0 nM 6.32
CHEMBL521806 GSK-237700A IC50 = 994.0 nM 6.00
CHEMBL514162 GW852849X IC50 = 1140.0 nM 5.94