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Assay Detail

CHEMBL984533

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Intrinsic inhibition of GST-fused human PDE4A expressed in SF9 cells
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

3',5'-cyclic-AMP phosphodiesterase 4A (CHEMBL254)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization and structure-activity relationship of a series of 1-phenyl-1,8-naphthyridin-4-one-3-carboxamides: identification of MK-0873, a potent and effective PDE4 inhibitor.
Guay D, Boulet L, Friesen RW, Girard M, Hamel P, Huang Z, Laliberté F, Laliberté S, Mancini JA, Muise E, Pon D, Styhler A.
Bioorg Med Chem Lett (2008) 18 : 5554 -5558
PubMed 18835163 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 8.68 10.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL499477 1 10.22
CHEMBL514564 1 9.77
CHEMBL519193 1 9.47
CHEMBL464804 1 9.43
CHEMBL456171 1 9.35
CHEMBL484753 1 9.21
CHEMBL485547 1 9.12
CHEMBL372575 1 8.85
CHEMBL456808 1 8.81
CHEMBL464600 1 8.45
CHEMBL485620 1 8.21
CHEMBL485629 MK-0873 2.0 1 8.17
CHEMBL456172 1 8.04
CHEMBL456173 1 7.82
CHEMBL511368 1 7.18
CHEMBL464803 1 6.85
CHEMBL464802 1 -
CHEMBL458106 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL499477 IC50 = 0.06 nM 10.22
CHEMBL514564 IC50 = 0.17 nM 9.77
CHEMBL519193 IC50 = 0.34 nM 9.47
CHEMBL464804 IC50 = 0.37 nM 9.43
CHEMBL456171 IC50 = 0.45 nM 9.35
CHEMBL484753 IC50 = 0.62 nM 9.21
CHEMBL485547 IC50 = 0.75 nM 9.12
CHEMBL372575 IC50 = 1.4 nM 8.85
CHEMBL456808 IC50 = 1.54 nM 8.81
CHEMBL464600 IC50 = 3.54 nM 8.45
CHEMBL485620 IC50 = 6.2 nM 8.21
CHEMBL485629 MK-0873 IC50 = 6.7 nM 8.17
CHEMBL456172 IC50 = 9.2 nM 8.04
CHEMBL456173 IC50 = 15.3 nM 7.82
CHEMBL511368 IC50 = 66.5 nM 7.18
CHEMBL464803 IC50 = 141.0 nM 6.85
CHEMBL464802 IC50 > 1000.0 nM -
CHEMBL458106 IC50 > 1000.0 nM -