Assay Detail
Functional
CHEMBL987095
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Inhibition of Hedgehog pathway-dependent oxysterol-induced mouse C3 H10T1/2 cell differentiation assessed as alkaline phosphatase production after 72 hrs
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Mus musculus |
| Cell Type | C3H 10T1/2 |
| Confidence | 3 — Cell-line / Tissue |
| Curated By | Autocuration |
Publication
Semisynthetic cyclopamine analogues as potent and orally bioavailable hedgehog pathway antagonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 13 | 6.87 | 8.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL449569 | — | — | 1 | 8.00 |
| CHEMBL506708 | — | — | 1 | 7.70 |
| CHEMBL489867 | — | — | 1 | 6.92 |
| CHEMBL254129 | CYCLOPAMINE | — | 1 | 6.77 |
| CHEMBL473393 | — | — | 1 | 6.70 |
| CHEMBL509596 | — | — | 1 | 6.40 |
| CHEMBL483601 | — | — | 1 | 6.40 |
| CHEMBL483765 | — | — | 1 | 6.10 |
| CHEMBL504507 | — | — | 1 | - |
| CHEMBL451346 | — | — | 1 | - |
| CHEMBL490068 | — | — | 1 | - |
| CHEMBL519497 | — | — | 1 | - |
| CHEMBL483591 | CYCLOPROPYL CYCLOPAMINE | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL449569 | — | EC50 | = | 10.0 | nM | 8.00 |
| CHEMBL506708 | — | EC50 | = | 20.0 | nM | 7.70 |
| CHEMBL489867 | — | EC50 | = | 120.0 | nM | 6.92 |
| CHEMBL254129 | CYCLOPAMINE | EC50 | = | 170.0 | nM | 6.77 |
| CHEMBL473393 | — | EC50 | = | 200.0 | nM | 6.70 |
| CHEMBL509596 | — | EC50 | = | 400.0 | nM | 6.40 |
| CHEMBL483601 | — | EC50 | = | 400.0 | nM | 6.40 |
| CHEMBL483765 | — | EC50 | = | 800.0 | nM | 6.10 |
| CHEMBL504507 | — | EC50 | > | 2000.0 | nM | - |
| CHEMBL451346 | — | EC50 | > | 1000.0 | nM | - |
| CHEMBL490068 | — | EC50 | > | 2500.0 | nM | - |
| CHEMBL519497 | — | EC50 | > | 2500.0 | nM | - |
| CHEMBL483591 | CYCLOPROPYL CYCLOPAMINE | EC50 | > | 2000.0 | nM | - |