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Assay Detail

CHEMBL1000350

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of large-conductance calcium-activated potassium channel-mediated vasorelaxation in endothelium-denuded normotensive Wistar rat thoracic aorta assessed as potassium chloride-induced contractile tension
18
Total Activities
18
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Thoracic aorta
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Calcium-activated potassium channel subunit alpha-1 (CHEMBL5505)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Novel 1,4-benzothiazine derivatives as large conductance Ca2+-activated potassium channel openers.
Calderone V, Spogli R, Martelli A, Manfroni G, Testai L, Sabatini S, Tabarrini O, Cecchetti V.
J Med Chem (2008) 51 : 5085 -5092
PubMed 18680269 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Activity 11 - -
IC50 7 5.41 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL496460 1 7.40
CHEMBL498516 1 6.21
CHEMBL384903 1 5.30
CHEMBL498702 1 5.20
CHEMBL525555 1 4.80
CHEMBL526824 1 4.65
CHEMBL525920 1 4.30
CHEMBL442643 1 -
CHEMBL497862 1 -
CHEMBL449286 1 -
CHEMBL497861 1 -
CHEMBL450728 1 -
CHEMBL498518 1 -
CHEMBL498502 1 -
CHEMBL496461 1 -
CHEMBL454472 1 -
CHEMBL507244 1 -
CHEMBL498517 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL496460 IC50 = 39.81 nM 7.40
CHEMBL498516 IC50 = 616.6 nM 6.21
CHEMBL384903 IC50 = 5011.87 nM 5.30
CHEMBL498702 IC50 = 6309.57 nM 5.20
CHEMBL525555 IC50 = 15848.93 nM 4.80
CHEMBL526824 IC50 = 22387.21 nM 4.65
CHEMBL525920 IC50 = 50118.72 nM 4.30
CHEMBL442643 Activity - -
CHEMBL497862 Activity - -
CHEMBL449286 Activity - -
CHEMBL497861 Activity - -
CHEMBL450728 Activity <= 50.0 % -
CHEMBL498518 Activity <= 50.0 % -
CHEMBL498502 Activity <= 50.0 % -
CHEMBL496461 Activity <= 50.0 % -
CHEMBL454472 Activity <= 50.0 % -
CHEMBL507244 Activity <= 50.0 % -
CHEMBL498517 Activity <= 50.0 % -