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Assay Detail

CHEMBL1019333

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Functional
Inhibition of protein translation in Escherichia coli by coupled transcription/translation assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Escherichia coli
Confidence 1 — Organism
Curated By Autocuration

Target

Escherichia coli (CHEMBL354)
Type ORGANISM
Organism Escherichia coli

Publication

Development of novel aminoglycoside (NB54) with reduced toxicity and enhanced suppression of disease-causing premature stop mutations.
Nudelman I, Rebibo-Sabbah A, Cherniavsky M, Belakhov V, Hainrichson M, Chen F, Schacht J, Pilch DS, Ben-Yosef T, Baasov T.
J Med Chem (2009) 52 : 2836 -2845
PubMed 19309154 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.03 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL6067485 GENTAMICIN 4.0 1 7.70
CHEMBL370143 PAROMOMYCIN 4.0 1 7.25
CHEMBL511656 1 6.80
CHEMBL474967 1 6.38

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL6067485 GENTAMICIN IC50 = 20.0 nM 7.70
CHEMBL370143 PAROMOMYCIN IC50 = 56.0 nM 7.25
CHEMBL511656 IC50 = 159.0 nM 6.80
CHEMBL474967 IC50 = 415.0 nM 6.38