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Assay Detail

CHEMBL1040025

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H](+)-pentazocine from sigma1 receptor in human Jurkat cell membrane
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Jurkat
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Sigma non-opioid intracellular receptor 1 (CHEMBL287)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel highly potent and selective sigma 1 receptor antagonists related to spipethiane.
Piergentili A, Amantini C, Del Bello F, Giannella M, Mattioli L, Palmery M, Perfumi M, Pigini M, Santoni G, Tucci P, Zotti M, Quaglia W.
J Med Chem (2010) 53 : 1261 -1269
PubMed 20067271 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 9.66 10.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL578825 1 10.28
CHEMBL570795 1 10.24
CHEMBL570529 1 10.18
CHEMBL571418 1 10.05
CHEMBL572294 1 10.01
CHEMBL570067 1 9.96
CHEMBL571659 1 9.68
CHEMBL570789 1 9.36
CHEMBL39900 SPIPETHIANE 1 9.23
CHEMBL38634 1 9.21
CHEMBL54 HALOPERIDOL 4.0 1 8.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL578825 Ki = 0.05248 nM 10.28
CHEMBL570795 Ki = 0.05754 nM 10.24
CHEMBL570529 Ki = 0.06607 nM 10.18
CHEMBL571418 Ki = 0.08913 nM 10.05
CHEMBL572294 Ki = 0.09772 nM 10.01
CHEMBL570067 Ki = 0.1096 nM 9.96
CHEMBL571659 Ki = 0.2089 nM 9.68
CHEMBL570789 Ki = 0.4365 nM 9.36
CHEMBL39900 SPIPETHIANE Ki = 0.5888 nM 9.23
CHEMBL38634 Ki = 0.6166 nM 9.21
CHEMBL54 HALOPERIDOL Ki = 7.762 nM 8.11